- 李健为,朱平
<正> 化疗是白血病治疗最常用的手段,它是根据细胞周期的原理设计的。化疗通过大量杀灭白血病细胞以求减少肿瘤细胞负荷,因此化疗剂量愈大,缓解愈彻底。遗憾的是化疗药物同样会影响正常细胞,限制了化疗的应用。通常理想的化疗是最大限度地杀灭了白血病细胞,而残留的正常造血干细胞仍然会增殖和分化来重建正常的造血系统。一般来说,只需要大约5％的正常骨髓干细胞就可以增殖为全部骨髓。由于大剂量的化疗可以完全摧毁患者的骨髓,
1997年03期 225-228页 [查看摘要][在线阅读][下载 268k] [下载次数：51 ] |[引用频次：0 ] |[阅读次数：0 ] - 李健为,朱平
<正> 化疗是白血病治疗最常用的手段,它是根据细胞周期的原理设计的。化疗通过大量杀灭白血病细胞以求减少肿瘤细胞负荷,因此化疗剂量愈大,缓解愈彻底。遗憾的是化疗药物同样会影响正常细胞,限制了化疗的应用。通常理想的化疗是最大限度地杀灭了白血病细胞,而残留的正常造血干细胞仍然会增殖和分化来重建正常的造血系统。一般来说,只需要大约5％的正常骨髓干细胞就可以增殖为全部骨髓。由于大剂量的化疗可以完全摧毁患者的骨髓,
1997年03期 225-228页 [查看摘要][在线阅读][下载 268k] [下载次数：51 ] |[引用频次：0 ] |[阅读次数：0 ] - 裴雪涛,王立生,徐黎,龙红,冯凯,吴祖泽
血小板生成素(Tpo)是巨核细胞增殖分化和血小板形成的主要调控因子。为了探讨Tpo对CD34~+造血祖细胞的协同扩增作用,我们利用免疫磁珠分离系统(MACS)分选出90.11％-97.57％纯度的脐带血CD34~+细胞,在周的体外液体培养体系中,观察了Tpo与干细胞因子(SCF),IL-3,IL-6,红细胞生成素(Epo),粒-巨噬细胞集落刺激因子(GM-CSF)和粒细胞集落刺激因子(G-CSF)不同组合的扩增效率。结果表明,Tpo具有明显的协同扩增作用,细胞总数、集落形成细胞(CFC)和CD34~+细胞可分别被新增329.50±25.20倍,16.09±3.38倍和11.77±4.78倍,SCF+IL-3+IL-6+Tpo组的扩增作用最强,含Tpo实验组的扩增效率明显高于不含Tpo组;同时,含Tpo的组合还使CFU-MK,CD41a~+细胞,BFU-E和CFU-GM分别扩增了34.67±4.62倍,17.29±2.34倍,14.97±2.89倍和14.46±3.19倍。表明Tpo具有明显的扩增造血细胞的作用和刺激多系造血的活性。
1997年03期 229-235页 [查看摘要][在线阅读][下载 282k] [下载次数：73 ] |[引用频次：19 ] |[阅读次数：0 ] - 裴雪涛,王立生,徐黎,龙红,冯凯,吴祖泽
血小板生成素(Tpo)是巨核细胞增殖分化和血小板形成的主要调控因子。为了探讨Tpo对CD34~+造血祖细胞的协同扩增作用,我们利用免疫磁珠分离系统(MACS)分选出90.11％-97.57％纯度的脐带血CD34~+细胞,在周的体外液体培养体系中,观察了Tpo与干细胞因子(SCF),IL-3,IL-6,红细胞生成素(Epo),粒-巨噬细胞集落刺激因子(GM-CSF)和粒细胞集落刺激因子(G-CSF)不同组合的扩增效率。结果表明,Tpo具有明显的协同扩增作用,细胞总数、集落形成细胞(CFC)和CD34~+细胞可分别被新增329.50±25.20倍,16.09±3.38倍和11.77±4.78倍,SCF+IL-3+IL-6+Tpo组的扩增作用最强,含Tpo实验组的扩增效率明显高于不含Tpo组;同时,含Tpo的组合还使CFU-MK,CD41a~+细胞,BFU-E和CFU-GM分别扩增了34.67±4.62倍,17.29±2.34倍,14.97±2.89倍和14.46±3.19倍。表明Tpo具有明显的扩增造血细胞的作用和刺激多系造血的活性。
1997年03期 229-235页 [查看摘要][在线阅读][下载 282k] [下载次数：73 ] |[引用频次：19 ] |[阅读次数：0 ] - 梅巍,贾志凌,杨科
应用重组血小板生成素(rTpo)对17例再生障碍性贫血(AA)患者进行CFU-MK和CFU-GM培养。研究结果表明:①Tpo 10ng/ml为CFU-MK培养的最佳浓度;②AA患者CFU-GM和CFU-MK增殖均明显低于正常人,P<0.001;③AA患者CFU-MK增殖较CFU-GM更低(CFU-MKx 3.2,CFU-GMx 17.5);④Tpo对骨髓小剂量照射后CFU-MK的增殖有促进恢复,对血小板的减少起到积极预防作用。
1997年03期 236-238页 [查看摘要][在线阅读][下载 163k] [下载次数：68 ] |[引用频次：1 ] |[阅读次数：0 ] - 梅巍,贾志凌,杨科
应用重组血小板生成素(rTpo)对17例再生障碍性贫血(AA)患者进行CFU-MK和CFU-GM培养。研究结果表明:①Tpo 10ng/ml为CFU-MK培养的最佳浓度;②AA患者CFU-GM和CFU-MK增殖均明显低于正常人,P<0.001;③AA患者CFU-MK增殖较CFU-GM更低(CFU-MKx 3.2,CFU-GMx 17.5);④Tpo对骨髓小剂量照射后CFU-MK的增殖有促进恢复,对血小板的减少起到积极预防作用。
1997年03期 236-238页 [查看摘要][在线阅读][下载 163k] [下载次数：68 ] |[引用频次：1 ] |[阅读次数：0 ] - 陆华中,邹萍,李崇渔
为探索一种安全、有效、简易的非病毒基因转移策略用于介导造血干细胞基因转移的可行性,本研究利用商品化的脂质体将两个标志基因(Neo R和Lac Z)共转导造血细胞。通过G418筛选、富集转导阳性细胞,观察了外源基因在小鼠原代骨髓细胞的基因转移率及其表达的稳定性。结果显示:通过脂质体介导,外源基因在小鼠骨髓细胞可获得有效的瞬时和稳定的表达。同时,经G418筛选,转导阳性细胞可得到明显的富集。提示利用脂质体这一非病毒载体个导造血干细胞基因转移的可行性。
1997年03期 239-242页 [查看摘要][在线阅读][下载 206k] [下载次数：22 ] |[引用频次：3 ] |[阅读次数：0 ] - 陆华中,邹萍,李崇渔
为探索一种安全、有效、简易的非病毒基因转移策略用于介导造血干细胞基因转移的可行性,本研究利用商品化的脂质体将两个标志基因(Neo R和Lac Z)共转导造血细胞。通过G418筛选、富集转导阳性细胞,观察了外源基因在小鼠原代骨髓细胞的基因转移率及其表达的稳定性。结果显示:通过脂质体介导,外源基因在小鼠骨髓细胞可获得有效的瞬时和稳定的表达。同时,经G418筛选,转导阳性细胞可得到明显的富集。提示利用脂质体这一非病毒载体个导造血干细胞基因转移的可行性。
1997年03期 239-242页 [查看摘要][在线阅读][下载 206k] [下载次数：22 ] |[引用频次：3 ] |[阅读次数：0 ] -
<正> 1997年7月7—10日在北京友谊宾馆召开了第1届北京国际基因治疗研讨会。会议有15个特邀专题报告和27个来自8个国家的研究报告。会议内容反映了当前国际基因治疗研究最前沿的先进水平,又指明了今后基因治疗研究的方向。专题报告人中有不少是国际著名的基因治疗领域的权威科学家,包括几届美国和欧洲基因治疗会议主席等。
1997年03期 242页 [查看摘要][在线阅读][下载 35k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] <正> 1997年7月7—10日在北京友谊宾馆召开了第1届北京国际基因治疗研讨会。会议有15个特邀专题报告和27个来自8个国家的研究报告。会议内容反映了当前国际基因治疗研究最前沿的先进水平,又指明了今后基因治疗研究的方向。专题报告人中有不少是国际著名的基因治疗领域的权威科学家,包括几届美国和欧洲基因治疗会议主席等。
1997年03期 242页 [查看摘要][在线阅读][下载 35k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] - 杜楠,王庆余,刘昕,章波,纪贤文
为了探索逆转录病毒介导的基因转移与鉴定方法,将含新霉素抗性(Neo R)基因的GM-CSF重组逆转录病毒转染病毒包装细胞PA317后,以NIH3T3细胞为靶细胞,通过体外克隆形成实验和原位PCR方法检测Neo R基因的转移效率。结果表明,原位PCR方法检测的基因转移效率(84.1%)明显高于前者(64%),它具有灵敏性高和特异性强,细胞内定位且标本用量少,省时及操作简便等特点。与标准PCR鉴定结果相符,同时,应用Southern blot,原位杂交和生物学方法检测转染细胞分别显示有GM-CSF cDNA整合、GM-CSF mRNA表达和GM-CSF生物学活性。上述结果表明重组质粒成功地整合在转染细胞基因组中并获得了表达。
1997年03期 243-248页 [查看摘要][在线阅读][下载 521k] [下载次数：24 ] |[引用频次：1 ] |[阅读次数：0 ] - 杜楠,王庆余,刘昕,章波,纪贤文
为了探索逆转录病毒介导的基因转移与鉴定方法,将含新霉素抗性(Neo R)基因的GM-CSF重组逆转录病毒转染病毒包装细胞PA317后,以NIH3T3细胞为靶细胞,通过体外克隆形成实验和原位PCR方法检测Neo R基因的转移效率。结果表明,原位PCR方法检测的基因转移效率(84.1%)明显高于前者(64%),它具有灵敏性高和特异性强,细胞内定位且标本用量少,省时及操作简便等特点。与标准PCR鉴定结果相符,同时,应用Southern blot,原位杂交和生物学方法检测转染细胞分别显示有GM-CSF cDNA整合、GM-CSF mRNA表达和GM-CSF生物学活性。上述结果表明重组质粒成功地整合在转染细胞基因组中并获得了表达。
1997年03期 243-248页 [查看摘要][在线阅读][下载 521k] [下载次数：24 ] |[引用频次：1 ] |[阅读次数：0 ] -
<正> 大会专题报告有: 骨髓移植后的免疫治疗 陆道培 人类基因组和白血病分化、凋亡的诱导 陈竺 FISH及染色体涂抹技术检测白血病染色体易位 陈赛娟
1997年03期 248页 [查看摘要][在线阅读][下载 46k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] <正> 大会专题报告有: 骨髓移植后的免疫治疗 陆道培 人类基因组和白血病分化、凋亡的诱导 陈竺 FISH及染色体涂抹技术检测白血病染色体易位 陈赛娟
1997年03期 248页 [查看摘要][在线阅读][下载 46k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] - 刘文励,周剑锋,肖侃艳,唐锦治
为了解p16基因在急性髓系白血病(AML)发病中的意义,用PCR-SSCP和Northern blot方法分析了16例AML细胞p16基因结构及mRNA的表达。研究结果表明,16例未发现p16基因的缺失、突变。初治和复发的12例AML的p16 mRNA表达水平明显低于4例长期缓解者及正常对照者。1例p53突变AML的p16 mRNA有明显升高,提示p16基因在AML发病中可能不占重要地位,而在AML细胞抑制正常造血的逐步恶化中起一定作用,在p53突变时,其表达反馈性升高显示一种反馈环路失衡。
1997年03期 249-252页 [查看摘要][在线阅读][下载 317k] [下载次数：13 ] |[引用频次：0 ] |[阅读次数：0 ] - 刘文励,周剑锋,肖侃艳,唐锦治
为了解p16基因在急性髓系白血病(AML)发病中的意义,用PCR-SSCP和Northern blot方法分析了16例AML细胞p16基因结构及mRNA的表达。研究结果表明,16例未发现p16基因的缺失、突变。初治和复发的12例AML的p16 mRNA表达水平明显低于4例长期缓解者及正常对照者。1例p53突变AML的p16 mRNA有明显升高,提示p16基因在AML发病中可能不占重要地位,而在AML细胞抑制正常造血的逐步恶化中起一定作用,在p53突变时,其表达反馈性升高显示一种反馈环路失衡。
1997年03期 249-252页 [查看摘要][在线阅读][下载 317k] [下载次数：13 ] |[引用频次：0 ] |[阅读次数：0 ] - 陈燕,杨年兰,向直富,李崇渔,王辨明
抑癌基因p53,p16的缺失和突变以及癌基因bcl-2/JH融合基因的出现是恶性淋巴瘤发生的分子生物学原因,p53和p16基因亦是诱导淋巴瘤细胞凋亡的重要基因,而bcl-2基因是抑制淋巴瘤细胞凋亡的重要基因。为研究这3种基因结构的变化,用PCR和PCR-SSCP,以及PCR扩增产物序列测定的方法测定了人9种恶性淋巴瘤细胞系,发现SU-DHL-1,SU-DHL-4,SU-DHL-6,SU-DHL-8,SU-DHL-10,Daudi,8392 7种恶性淋巴瘤细胞系有p53基因的点突变,分别在第五、六、七外显子;SU-DHL-9有p16基因的纯合缺失,而SU-DHL-1和Daudi细胞系有p16基因的第二外显子的突变,SU-DHL-1的测序结果为密码子30的GAC突变为AAC,密码子35的GCT突变为ACT,密码子40与41之间插入了一个C;SU-DHL-4和SU-DHL-6有bcl-2/JH基因的重排,讨论了基因结构的变化在恶性淋巴瘤细胞系发生发展中的意义。
1997年03期 253-258页 [查看摘要][在线阅读][下载 340k] [下载次数：56 ] |[引用频次：2 ] |[阅读次数：0 ] - 陈燕,杨年兰,向直富,李崇渔,王辨明
抑癌基因p53,p16的缺失和突变以及癌基因bcl-2/JH融合基因的出现是恶性淋巴瘤发生的分子生物学原因,p53和p16基因亦是诱导淋巴瘤细胞凋亡的重要基因,而bcl-2基因是抑制淋巴瘤细胞凋亡的重要基因。为研究这3种基因结构的变化,用PCR和PCR-SSCP,以及PCR扩增产物序列测定的方法测定了人9种恶性淋巴瘤细胞系,发现SU-DHL-1,SU-DHL-4,SU-DHL-6,SU-DHL-8,SU-DHL-10,Daudi,8392 7种恶性淋巴瘤细胞系有p53基因的点突变,分别在第五、六、七外显子;SU-DHL-9有p16基因的纯合缺失,而SU-DHL-1和Daudi细胞系有p16基因的第二外显子的突变,SU-DHL-1的测序结果为密码子30的GAC突变为AAC,密码子35的GCT突变为ACT,密码子40与41之间插入了一个C;SU-DHL-4和SU-DHL-6有bcl-2/JH基因的重排,讨论了基因结构的变化在恶性淋巴瘤细胞系发生发展中的意义。
1997年03期 253-258页 [查看摘要][在线阅读][下载 340k] [下载次数：56 ] |[引用频次：2 ] |[阅读次数：0 ] - 张锑,张春青,纪春岩,刘传方,乔颖,徐从高
多药耐药(MDR)是急性白血病化疗成功的主要障碍之一,其机理涉及诸多因素。建立能够体外准确预测化疗药物敏感性试验方法,在指导急性白血病治疗中具有重要意义。本文研究了42例急性白血病患者体外药敏检测(MTT法)和白血病细胞P-gp)抗原的表达与白血病化疗疗效的关系。结果显示:药敏试验与治疗疗效有相关性,临床总符合率82.9%,阳性符合率78.9％,阴性符合率86.9％。认为P-gp的表达是一个有效的化疗耐药指标,白血病缓解病人P-gp阳性率高于初诊病人,P-gp阳性表达的患者完全缓解率明显低于P-gp阴性患者。
1997年03期 259-262页 [查看摘要][在线阅读][下载 198k] [下载次数：39 ] |[引用频次：4 ] |[阅读次数：0 ] - 张锑,张春青,纪春岩,刘传方,乔颖,徐从高
多药耐药(MDR)是急性白血病化疗成功的主要障碍之一,其机理涉及诸多因素。建立能够体外准确预测化疗药物敏感性试验方法,在指导急性白血病治疗中具有重要意义。本文研究了42例急性白血病患者体外药敏检测(MTT法)和白血病细胞P-gp)抗原的表达与白血病化疗疗效的关系。结果显示:药敏试验与治疗疗效有相关性,临床总符合率82.9%,阳性符合率78.9％,阴性符合率86.9％。认为P-gp的表达是一个有效的化疗耐药指标,白血病缓解病人P-gp阳性率高于初诊病人,P-gp阳性表达的患者完全缓解率明显低于P-gp阴性患者。
1997年03期 259-262页 [查看摘要][在线阅读][下载 198k] [下载次数：39 ] |[引用频次：4 ] |[阅读次数：0 ] - 刘蕙珍,陈怡祥
观察了全反式维甲酸(ATRA)对人类多发性骨髓瘤(MM)OPM-2细胞系作用,~3H-TdR掺入法显示ATRA使S及G_2/M期细胞标记指数下降,提示G_1→S期细胞增殖受抑制。抑制程度与其浓度呈依赖关系。当ATRA浓度在≤(1-2)×10~(-8)mol/L有时反有刺激生长的作用。外源性IL-6(rhIL-6)能刺激OPM-2细胞克隆生长,抗IL-6单克隆抗体(McAb)可抑制其生长,rhIL-6不能逆转ATRA致OPM-2细胞生长抑制,提示OPM-2细胞能自分泌IL-6。~(125)I标记IL-6测得细胞表面IL-6受体(IL-6R)密度降低及亲和性下降,RT-PCR测得IL-6RmRNA下降,但gp130 mRNA表达无改变,在培养上清液测不出IL-6活性。这进一步证明ATRA对OPM-2细胞增殖的抑制作用是通过下调IL-6R及抑制瘤细胞自分泌IL-6双重作用而实现的。本实验提出ATRA有可能作为一种新的抗增殖药物治疗MM及其它自/旁分泌IL-6依赖性生长特性的肿瘤。
1997年03期 263-268页 [查看摘要][在线阅读][下载 273k] [下载次数：55 ] |[引用频次：4 ] |[阅读次数：0 ] - 刘蕙珍,陈怡祥
观察了全反式维甲酸(ATRA)对人类多发性骨髓瘤(MM)OPM-2细胞系作用,~3H-TdR掺入法显示ATRA使S及G_2/M期细胞标记指数下降,提示G_1→S期细胞增殖受抑制。抑制程度与其浓度呈依赖关系。当ATRA浓度在≤(1-2)×10~(-8)mol/L有时反有刺激生长的作用。外源性IL-6(rhIL-6)能刺激OPM-2细胞克隆生长,抗IL-6单克隆抗体(McAb)可抑制其生长,rhIL-6不能逆转ATRA致OPM-2细胞生长抑制,提示OPM-2细胞能自分泌IL-6。~(125)I标记IL-6测得细胞表面IL-6受体(IL-6R)密度降低及亲和性下降,RT-PCR测得IL-6RmRNA下降,但gp130 mRNA表达无改变,在培养上清液测不出IL-6活性。这进一步证明ATRA对OPM-2细胞增殖的抑制作用是通过下调IL-6R及抑制瘤细胞自分泌IL-6双重作用而实现的。本实验提出ATRA有可能作为一种新的抗增殖药物治疗MM及其它自/旁分泌IL-6依赖性生长特性的肿瘤。
1997年03期 263-268页 [查看摘要][在线阅读][下载 273k] [下载次数：55 ] |[引用频次：4 ] |[阅读次数：0 ] - 杨晶,刘作斌,汪月增
探讨了冷冻保存对骨髓单个核细胞分泌IL-1和IL-6的影响。冻存后细胞经有丝分裂原(LPS)刺激后培养上清中的IL-1和IL-6的含量均显著高于新鲜细胞。细胞表面IL-1和IL-6抗体平均荧光强度表达冻后细胞明显高于新鲜细胞。实验结果提示冷冻对抑制性单核细胞的灭活促进了细胞IL-1和IL-6的分泌。冻后细胞的这一状态有利于机体免疫功能的重建。
1997年03期 269-273页 [查看摘要][在线阅读][下载 230k] [下载次数：37 ] |[引用频次：3 ] |[阅读次数：0 ] - 杨晶,刘作斌,汪月增
探讨了冷冻保存对骨髓单个核细胞分泌IL-1和IL-6的影响。冻存后细胞经有丝分裂原(LPS)刺激后培养上清中的IL-1和IL-6的含量均显著高于新鲜细胞。细胞表面IL-1和IL-6抗体平均荧光强度表达冻后细胞明显高于新鲜细胞。实验结果提示冷冻对抑制性单核细胞的灭活促进了细胞IL-1和IL-6的分泌。冻后细胞的这一状态有利于机体免疫功能的重建。
1997年03期 269-273页 [查看摘要][在线阅读][下载 230k] [下载次数：37 ] |[引用频次：3 ] |[阅读次数：0 ] - 韩永胜,郭秀枝,李扬秋,汪明春
<正> 干细胞白血病(stem cell leukemia,SCL)基因是具有碱性螺旋-环-螺旋(basic helix-loop-helix bHLH)结构的转录因子家族成员之一,其功能与造血细胞的增殖、分化及T细胞肿瘤的发生有关。本文应用逆转录聚合酶链反应(RT-PCR)检测了SCL基因在白血病细胞中的表达情况,并对其意义作了初步探讨。
1997年03期 274-275页 [查看摘要][在线阅读][下载 134k] [下载次数：41 ] |[引用频次：0 ] |[阅读次数：0 ] - 韩永胜,郭秀枝,李扬秋,汪明春
<正> 干细胞白血病(stem cell leukemia,SCL)基因是具有碱性螺旋-环-螺旋(basic helix-loop-helix bHLH)结构的转录因子家族成员之一,其功能与造血细胞的增殖、分化及T细胞肿瘤的发生有关。本文应用逆转录聚合酶链反应(RT-PCR)检测了SCL基因在白血病细胞中的表达情况,并对其意义作了初步探讨。
1997年03期 274-275页 [查看摘要][在线阅读][下载 134k] [下载次数：41 ] |[引用频次：0 ] |[阅读次数：0 ] - 张双喜,孙启鸿,陈勇,孙瑛勋,于鸣,沈倍奋
<正>定性、定位和定量地确定细胞表达的各种重要分子是在分子水平上认识细胞功能的前提,其中以确定特定分子在细胞膜表面的绝对数目较为困难。目前多以放射性核素标记其配
1997年03期 276-280页 [查看摘要][在线阅读][下载 160k] [下载次数：227 ] |[引用频次：0 ] |[阅读次数：0 ] - 张双喜,孙启鸿,陈勇,孙瑛勋,于鸣,沈倍奋
<正>定性、定位和定量地确定细胞表达的各种重要分子是在分子水平上认识细胞功能的前提,其中以确定特定分子在细胞膜表面的绝对数目较为困难。目前多以放射性核素标记其配
1997年03期 276-280页 [查看摘要][在线阅读][下载 160k] [下载次数：227 ] |[引用频次：0 ] |[阅读次数：0 ] - Thomas Blankenstein
<正> Cytokines provided locally at tbe tumor site mayinitiate an effective anti-tumor immune responsewhich leads to rejection of a tumor which otherwisegrows progressively. Experimentillly, this can betested by gene transfer into cultured tumor cellsfollowed by the analysis of the tumorigenicity of suchgenetically engincered cells. This approach allows to
1997年03期 281页 [查看摘要][在线阅读][下载 38k] [下载次数：7 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Since 1980, many Chinese scientists haveinvestigated the somatic cell therapy and started thebasic research on gene therapy. From 1990' s, someimportant advances in gene therapy research havebeen achieved in China. For example, the clinicaltrial of hemophilia B was approved in China andpublished in Human Gene Therapy. Many originalpapers have been published in the internationaljournals. Many gene therapy research projects havebeen funded by the National N atural ScienceFoundation and National High BiotechnologyFoundation. The National High BiotechnologyFoundation has supportcd 10 gene therapy research
1997年03期 281-282页 [查看摘要][在线阅读][下载 88k] [下载次数：13 ] |[引用频次：0 ] |[阅读次数：0 ] - Thomas Blankenstein
<正> Cytokines provided locally at tbe tumor site mayinitiate an effective anti-tumor immune responsewhich leads to rejection of a tumor which otherwisegrows progressively. Experimentillly, this can betested by gene transfer into cultured tumor cellsfollowed by the analysis of the tumorigenicity of suchgenetically engincered cells. This approach allows to
1997年03期 281页 [查看摘要][在线阅读][下载 38k] [下载次数：7 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Since 1980, many Chinese scientists haveinvestigated the somatic cell therapy and started thebasic research on gene therapy. From 1990' s, someimportant advances in gene therapy research havebeen achieved in China. For example, the clinicaltrial of hemophilia B was approved in China andpublished in Human Gene Therapy. Many originalpapers have been published in the internationaljournals. Many gene therapy research projects havebeen funded by the National N atural ScienceFoundation and National High BiotechnologyFoundation. The National High BiotechnologyFoundation has supportcd 10 gene therapy research
1997年03期 281-282页 [查看摘要][在线阅读][下载 88k] [下载次数：13 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Optimal activation of T cells requires at least 2signals. Signal one is generated by interactionsbetween T cell receptor and antigenic peptide-majorhistocompatibility complex on antigen-presentingcells. Signal two is delivered by co-stimulatory ligandson antigen-presenting cells to their receptors on T
1997年03期 282页 [查看摘要][在线阅读][下载 50k] [下载次数：7 ] |[引用频次：0 ] |[阅读次数：0 ] - Albert B.Deisseroth
<正> Cancer gene therapy over the past several yearshas involved the introduction of genes intohematopoietic cells for: (1) protecting the normalcells from the side effects of chemotherapy; (2)introduction of genes into the neoplastic cells for the
1997年03期 282-283页 [查看摘要][在线阅读][下载 101k] [下载次数：6 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Optimal activation of T cells requires at least 2signals. Signal one is generated by interactionsbetween T cell receptor and antigenic peptide-majorhistocompatibility complex on antigen-presentingcells. Signal two is delivered by co-stimulatory ligandson antigen-presenting cells to their receptors on T
1997年03期 282页 [查看摘要][在线阅读][下载 50k] [下载次数：7 ] |[引用频次：0 ] |[阅读次数：0 ] - Albert B.Deisseroth
<正> Cancer gene therapy over the past several yearshas involved the introduction of genes intohematopoietic cells for: (1) protecting the normalcells from the side effects of chemotherapy; (2)introduction of genes into the neoplastic cells for the
1997年03期 282-283页 [查看摘要][在线阅读][下载 101k] [下载次数：6 ] |[引用频次：0 ] |[阅读次数：0 ] - J.C.Gloriosc,D.J.Fink
<正> Herpes simplex virus type 1 (HSV-1) naturallyestablishes latency in neurons of the nervous systemwith the concommitant expression of the latencyassociated transcripts (LATS) and the loss in lytic
1997年03期 283页 [查看摘要][在线阅读][下载 52k] [下载次数：8 ] |[引用频次：0 ] |[阅读次数：0 ] - Philip Greenberg,C.Yee,M.Gilbert,B.Nelson,J.Lord,A.Sing,M.Kalos,S.Riddell
<正> Our laboratory has pursued T cell therapy withantigen-specific T cell clones as means to treat humandisease. Our studies in the prevention of CMV disease
1997年03期 283-284页 [查看摘要][在线阅读][下载 105k] [下载次数：15 ] |[引用频次：0 ] |[阅读次数：0 ] - J.C.Gloriosc,D.J.Fink
<正> Herpes simplex virus type 1 (HSV-1) naturallyestablishes latency in neurons of the nervous systemwith the concommitant expression of the latencyassociated transcripts (LATS) and the loss in lytic
1997年03期 283页 [查看摘要][在线阅读][下载 52k] [下载次数：8 ] |[引用频次：0 ] |[阅读次数：0 ] - Philip Greenberg,C.Yee,M.Gilbert,B.Nelson,J.Lord,A.Sing,M.Kalos,S.Riddell
<正> Our laboratory has pursued T cell therapy withantigen-specific T cell clones as means to treat humandisease. Our studies in the prevention of CMV disease
1997年03期 283-284页 [查看摘要][在线阅读][下载 105k] [下载次数：15 ] |[引用频次：0 ] |[阅读次数：0 ] - Y.J.Guo,F.Shen,T.P.Xie,X.Che,Z.F.Cui,L.Shi,J.Ma,S.G.Wu,X.N.Wang,G.L.Liu,Y.Liu,H.Wang,H.L.Huang,L.X.Wei,J.Zhao,J.Trojan,A.Ly,D.Anthony,M.C.Wu
<正> Tumor cells escape host immune surveillance bydown-regulation of MHC and/or co-stimulatorymolecules. Anti-tumor immune responses are
1997年03期 284-285页 [查看摘要][在线阅读][下载 105k] [下载次数：18 ] |[引用频次：0 ] |[阅读次数：0 ] - Y.J.Guo,F.Shen,T.P.Xie,X.Che,Z.F.Cui,L.Shi,J.Ma,S.G.Wu,X.N.Wang,G.L.Liu,Y.Liu,H.Wang,H.L.Huang,L.X.Wei,J.Zhao,J.Trojan,A.Ly,D.Anthony,M.C.Wu
<正> Tumor cells escape host immune surveillance bydown-regulation of MHC and/or co-stimulatorymolecules. Anti-tumor immune responses are
1997年03期 284-285页 [查看摘要][在线阅读][下载 105k] [下载次数：18 ] |[引用频次：0 ] |[阅读次数：0 ] - Nagy A.Habib,FRCS,ChM
<正> Liver tumours are among the most commonmalignancies worldwide. The international incidenceof the disease is about l million cases per year (male:femalc, 4: 1 ). These include primary and secondarytumours. Cancer is known to be a genetic disease thatinvolves the alteration of activity of the products of atleast 2 genes, due to mutation (s) and/or otherfactors, e. g., the presence of viral oncoproteins. Thegenes most affected are the tumour suppressor genes
1997年03期 285-286页 [查看摘要][在线阅读][下载 104k] [下载次数：15 ] |[引用频次：0 ] |[阅读次数：0 ] - Nagy A.Habib,FRCS,ChM
<正> Liver tumours are among the most commonmalignancies worldwide. The international incidenceof the disease is about l million cases per year (male:femalc, 4: 1 ). These include primary and secondarytumours. Cancer is known to be a genetic disease thatinvolves the alteration of activity of the products of atleast 2 genes, due to mutation (s) and/or otherfactors, e. g., the presence of viral oncoproteins. Thegenes most affected are the tumour suppressor genes
1997年03期 285-286页 [查看摘要][在线阅读][下载 104k] [下载次数：15 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Retroviral mediated gene transfer of humanglobin gene into hematopoietic stem cells is apromising approach for thalassemia gene therapy.Major problem of the transferred globin gene was lowlevel expression of the gene with its proximal cis-acting sequence. The locus control region (LCR) ofthe human β-globin gene cluster consists of four majorDNase I hypersensitive sites (HS). When linked to
1997年03期 286页 [查看摘要][在线阅读][下载 53k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] - Beverly S.Mitchell M.D.
<正> Utilization of gene therapy approaches for cancertreatment requires either that the transferred genegains access to the great majority of the tumor cells orthat gene transfer results in a cytotoxic effect that willkill a large number of tumor cells that do not directlyreceive the gene of interest. The latter effect can beachieved by the transfer into tumors of specific
1997年03期 286-287页 [查看摘要][在线阅读][下载 102k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] - Beverly S.Mitchell M.D.
<正> Utilization of gene therapy approaches for cancertreatment requires either that the transferred genegains access to the great majority of the tumor cells orthat gene transfer results in a cytotoxic effect that willkill a large number of tumor cells that do not directlyreceive the gene of interest. The latter effect can beachieved by the transfer into tumors of specific
1997年03期 286-287页 [查看摘要][在线阅读][下载 102k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Retroviral mediated gene transfer of humanglobin gene into hematopoietic stem cells is apromising approach for thalassemia gene therapy.Major problem of the transferred globin gene was lowlevel expression of the gene with its proximal cis-acting sequence. The locus control region (LCR) ofthe human β-globin gene cluster consists of four majorDNase I hypersensitive sites (HS). When linked to
1997年03期 286页 [查看摘要][在线阅读][下载 53k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] - J.Trojan,H.T.Duc,L.C.UpeguiGonzalez,B.Swiercz,F.Hor,D.D.Anthony,I.Royston
<正> The insulin-like growth factor l (IGF-1) iscommonly expressed in most of tumor cells and plays arole in the growth and development of animal andhuman tumors. Tumor cells transfected with a vectorencoding antisense IGF-l cDNA transcriptionalcassette driven by the mouse metallothionein-l
1997年03期 287页 [查看摘要][在线阅读][下载 49k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] - Robert E.Sobol M.D.
<正> The essence of gene therapy is to placefunctioning genes into a patient's cells for therapeuticpurposes. Gene therapy may be employed to ①enhance anti-tumor immune responses; ② correct
1997年03期 287-288页 [查看摘要][在线阅读][下载 104k] [下载次数：18 ] |[引用频次：0 ] |[阅读次数：0 ] - J.Trojan,H.T.Duc,L.C.UpeguiGonzalez,B.Swiercz,F.Hor,D.D.Anthony,I.Royston
<正> The insulin-like growth factor l (IGF-1) iscommonly expressed in most of tumor cells and plays arole in the growth and development of animal andhuman tumors. Tumor cells transfected with a vectorencoding antisense IGF-l cDNA transcriptionalcassette driven by the mouse metallothionein-l
1997年03期 287页 [查看摘要][在线阅读][下载 49k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] - Robert E.Sobol M.D.
<正> The essence of gene therapy is to placefunctioning genes into a patient's cells for therapeuticpurposes. Gene therapy may be employed to ①enhance anti-tumor immune responses; ② correct
1997年03期 287-288页 [查看摘要][在线阅读][下载 104k] [下载次数：18 ] |[引用频次：0 ] |[阅读次数：0 ] - Peter Walden,Uwe Trefzer,Wolfram Sterry,Gernot Stuhler
<正> Many cancer gene therapy strategies arevaccination strategies aiming at the induction ofcytolytic immune responses against the malignantcells. These strategies include modification of thetumour cells by transfection with the genes of co-stimulatory molecules for cytotoxic T lymphocytes(CTL) such as IL-2 and B7, or manipulation ofbystander cells like autologous fibroblasts byintroduction of cytokine genes and subsequent co-
1997年03期 288-289页 [查看摘要][在线阅读][下载 107k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] - Peter Walden,Uwe Trefzer,Wolfram Sterry,Gernot Stuhler
<正> Many cancer gene therapy strategies arevaccination strategies aiming at the induction ofcytolytic immune responses against the malignantcells. These strategies include modification of thetumour cells by transfection with the genes of co-stimulatory molecules for cytotoxic T lymphocytes(CTL) such as IL-2 and B7, or manipulation ofbystander cells like autologous fibroblasts byintroduction of cytokine genes and subsequent co-
1997年03期 288-289页 [查看摘要][在线阅读][下载 107k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] - Nelson A.Wivel
<正> It has been approximately six and one-half yearssince the first patient was enrolled in a gene therapyclinical trial. Since that important begining, this typeof genetic intervention has grown into a worldwideinvestigative activity involving about 2100 patients asof December 1996. Although it was initially thought
1997年03期 289-290页 [查看摘要][在线阅读][下载 107k] [下载次数：11 ] |[引用频次：0 ] |[阅读次数：0 ] - Nelson A.Wivel
<正> It has been approximately six and one-half yearssince the first patient was enrolled in a gene therapyclinical trial. Since that important begining, this typeof genetic intervention has grown into a worldwideinvestigative activity involving about 2100 patients asof December 1996. Although it was initially thought
1997年03期 289-290页 [查看摘要][在线阅读][下载 107k] [下载次数：11 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Hemophilia B, affecting 1 in 30000 males, is aserious X-linked recessive bleeding disorder due to apartial or complete deficiency of human clottingfactor Ⅸ (hFⅨ) activity in the plasma. Conventionaltherapy depends on the infusion of human plasma orconcentrate. The therapeutic effect is temporary and
1997年03期 290-292页 [查看摘要][在线阅读][下载 155k] [下载次数：12 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Hemophilia B, affecting 1 in 30000 males, is aserious X-linked recessive bleeding disorder due to apartial or complete deficiency of human clottingfactor Ⅸ (hFⅨ) activity in the plasma. Conventionaltherapy depends on the infusion of human plasma orconcentrate. The therapeutic effect is temporary and
1997年03期 290-292页 [查看摘要][在线阅读][下载 155k] [下载次数：12 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> The elimination of the tumor is closely relatedwith the sensitivity of tumor cells to the cytotoxicityof immune effector cells. We supposed that cytokinegenetransfection may increase the cytotoxicitysusceptibility of tumor cells to effector cells, and as aconsequence, the tumorigenicity decreased. Beforekilling tumor cells, effector cells required first torecognize non-specific surface adhesion molecules on
1997年03期 292页 [查看摘要][在线阅读][下载 46k] [下载次数：11 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Macrophage inflammatory protein-l, a recentlycharacterized chemokine, consists of two chains (αand β). MIP-lα has been shown to exert strongchemotactic effect on neutrophils, monocytes and Tlymphocytes. In the present study, the B16 melanomacells were transfected with recombinant adenoviruscontaining MIP-lα gene. The biological characteri-zation of the MIP-1α gene transfected B16 melanomacells was investigated. The level of MIP-1α in thesupernatant of gene-transfected melanoma cells was368±24 ng/ml/10~6/24hr.. By using Boyden chambersystem, this supernatant showed strong chemotacticactivity for NK cells, CD4~+ T cells, CD8~+ T cells orthe freshly isolated peritoneal macrophages in vitro.Though the in vitro growth of the gene-transfected B16 melanoma cclls was not aftered, the in vivogrowth of the tumor cells subcutaneously inoculatedwas significantly inhibited. The infiltration ofinflammatory cells into the tumor mass formed bygene-transfected B16 cells was much more obviousthan that by wild-type
1997年03期 292-293页 [查看摘要][在线阅读][下载 96k] [下载次数：15 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> The elimination of the tumor is closely relatedwith the sensitivity of tumor cells to the cytotoxicityof immune effector cells. We supposed that cytokinegenetransfection may increase the cytotoxicitysusceptibility of tumor cells to effector cells, and as aconsequence, the tumorigenicity decreased. Beforekilling tumor cells, effector cells required first torecognize non-specific surface adhesion molecules on
1997年03期 292页 [查看摘要][在线阅读][下载 46k] [下载次数：11 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Macrophage inflammatory protein-l, a recentlycharacterized chemokine, consists of two chains (αand β). MIP-lα has been shown to exert strongchemotactic effect on neutrophils, monocytes and Tlymphocytes. In the present study, the B16 melanomacells were transfected with recombinant adenoviruscontaining MIP-lα gene. The biological characteri-zation of the MIP-1α gene transfected B16 melanomacells was investigated. The level of MIP-1α in thesupernatant of gene-transfected melanoma cells was368±24 ng/ml/10~6/24hr.. By using Boyden chambersystem, this supernatant showed strong chemotacticactivity for NK cells, CD4~+ T cells, CD8~+ T cells orthe freshly isolated peritoneal macrophages in vitro.Though the in vitro growth of the gene-transfected B16 melanoma cclls was not aftered, the in vivogrowth of the tumor cells subcutaneously inoculatedwas significantly inhibited. The infiltration ofinflammatory cells into the tumor mass formed bygene-transfected B16 cells was much more obviousthan that by wild-type
1997年03期 292-293页 [查看摘要][在线阅读][下载 96k] [下载次数：15 ] |[引用频次：0 ] |[阅读次数：0 ] - Poornima Kamath,Edward J.Shillitoe,Karen Adler-Storthz
<正> Gene therapy of human cancer is likely to bemost effective when it is directed at targets that areexpressed in cancer cells but are lacking from othercells. Human papillomaviruses (HPV) can providesuch target, since these viruses are present in manycervical and oral cancers, and likely to be etiologicagents of the tumor. The oncogenic ability of HPVhas been assigned primarily to its two early genes, E6and E7. Continued expression of these two genes is
1997年03期 293页 [查看摘要][在线阅读][下载 51k] [下载次数：17 ] |[引用频次：0 ] |[阅读次数：0 ] - Poornima Kamath,Lisa St.John,Karen Adler-Storthz,Edward J.Shillitoe,Eric P.Gilchrist
<正> Hammerhead ribozymes are small RNA moleculesthat bind to a complementary sequence of RNA andcleave that sequence at a conserved triplet NUX (N =G, A, X = A, U, C). ln comparison with ilntisensemolecuIes, ribozymes not only bind to target RNA butalso cleave the target at predicted sites. They havebeen used as molecular agents to destroy either viralRNA or oncogene transcripts in human cancers. Manyhuman cervical and oral carcinomas express RNA of
1997年03期 293-294页 [查看摘要][在线阅读][下载 104k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] - Poornima Kamath,Edward J.Shillitoe,Karen Adler-Storthz
<正> Gene therapy of human cancer is likely to bemost effective when it is directed at targets that areexpressed in cancer cells but are lacking from othercells. Human papillomaviruses (HPV) can providesuch target, since these viruses are present in manycervical and oral cancers, and likely to be etiologicagents of the tumor. The oncogenic ability of HPVhas been assigned primarily to its two early genes, E6and E7. Continued expression of these two genes is
1997年03期 293页 [查看摘要][在线阅读][下载 51k] [下载次数：17 ] |[引用频次：0 ] |[阅读次数：0 ] - Poornima Kamath,Lisa St.John,Karen Adler-Storthz,Edward J.Shillitoe,Eric P.Gilchrist
<正> Hammerhead ribozymes are small RNA moleculesthat bind to a complementary sequence of RNA andcleave that sequence at a conserved triplet NUX (N =G, A, X = A, U, C). ln comparison with ilntisensemolecuIes, ribozymes not only bind to target RNA butalso cleave the target at predicted sites. They havebeen used as molecular agents to destroy either viralRNA or oncogene transcripts in human cancers. Manyhuman cervical and oral carcinomas express RNA of
1997年03期 293-294页 [查看摘要][在线阅读][下载 104k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Objective: Overexpression of the membraneepidermal growth factor receptor (EGFR) in humantumor cells predicts for poor prognosis. Hence, weexperimentally investigated the effects of humanEGFR to the malignant phenotype in humen breastcancer cell line MDA-MB-231 and humannasopharyngeal carcinoma cell line CNE-2. Methods:
1997年03期 294页 [查看摘要][在线阅读][下载 53k] [下载次数：11 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Objectives:To isolate tumor metastaticsuppressing genes or relating human DNA sequencesand to study the molecular biological regulatingmechanism of tumor metastasis. Methods: HumanDNA fragments were amplified by Inter Alu PCRtechnique from normal human genomic DNAtransfected mouse tumor cell clones of which themetastatic phenotype had been suppressed. Thehuman origin of the amplified DNA was furtherconfirmed by PCR in situ hybridization. One DNA
1997年03期 294-295页 [查看摘要][在线阅读][下载 104k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Objective: Overexpression of the membraneepidermal growth factor receptor (EGFR) in humantumor cells predicts for poor prognosis. Hence, weexperimentally investigated the effects of humanEGFR to the malignant phenotype in humen breastcancer cell line MDA-MB-231 and humannasopharyngeal carcinoma cell line CNE-2. Methods:
1997年03期 294页 [查看摘要][在线阅读][下载 53k] [下载次数：11 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Objectives:To isolate tumor metastaticsuppressing genes or relating human DNA sequencesand to study the molecular biological regulatingmechanism of tumor metastasis. Methods: HumanDNA fragments were amplified by Inter Alu PCRtechnique from normal human genomic DNAtransfected mouse tumor cell clones of which themetastatic phenotype had been suppressed. Thehuman origin of the amplified DNA was furtherconfirmed by PCR in situ hybridization. One DNA
1997年03期 294-295页 [查看摘要][在线阅读][下载 104k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> In recent years, great enthusiasm is given to theresearches of cancer gene therapy, which seems to bea promising novel strategy for cancer treatment.There are several strategies to deliver target genes intobodies. Among them, adenovirus-mediated genetransfection exhibits great advantages including highgene transfer efficiency and high expression level. Butthe optimal route of gene delivery is still uncertain.
1997年03期 295-296页 [查看摘要][在线阅读][下载 104k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] <正> In recent years, great enthusiasm is given to theresearches of cancer gene therapy, which seems to bea promising novel strategy for cancer treatment.There are several strategies to deliver target genes intobodies. Among them, adenovirus-mediated genetransfection exhibits great advantages including highgene transfer efficiency and high expression level. Butthe optimal route of gene delivery is still uncertain.
1997年03期 295-296页 [查看摘要][在线阅读][下载 104k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Numerous animal experiments and clinical trialshave demonstrated that the effects of systemicantitumor immune responses are closely correlatedwith the infiltration of effector cells, e. g.macrophages, T cells and NK cells, at the site oftumor. In previous studies, we have established theIL-2, IL-4, IL-6 gene-transfected B16F10 melanoma
1997年03期 296页 [查看摘要][在线阅读][下载 53k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Several features of adenoviruses make them anattractive option as a vehicle to transfer genes intoprimary malignant neoplasms in vivo. These viruseshave low pathogenicity in humans and are notneurotoxic. In addition, high titers of the virus can beachieved to allow higher levels of gene transfectionefficiency than the other vector systems. In vivotumorigenicity of G422 glioblastoma cells transfectedwith IL-2 and/or IL-3 genes decreased significantly inour privious report. In this study, recombinantadenoviruses were used to evaluate the therapeuticpotential of combined IL-2/IL-3 gene therapy in thetreatment of established subcutaneous tumor model ofG422 glioblastoma. Murine IL-2, IL-3 recombinantadenoviruses (2×10~8 pfu) were injected directly into
1997年03期 296-297页 [查看摘要][在线阅读][下载 104k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Numerous animal experiments and clinical trialshave demonstrated that the effects of systemicantitumor immune responses are closely correlatedwith the infiltration of effector cells, e. g.macrophages, T cells and NK cells, at the site oftumor. In previous studies, we have established theIL-2, IL-4, IL-6 gene-transfected B16F10 melanoma
1997年03期 296页 [查看摘要][在线阅读][下载 53k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Several features of adenoviruses make them anattractive option as a vehicle to transfer genes intoprimary malignant neoplasms in vivo. These viruseshave low pathogenicity in humans and are notneurotoxic. In addition, high titers of the virus can beachieved to allow higher levels of gene transfectionefficiency than the other vector systems. In vivotumorigenicity of G422 glioblastoma cells transfectedwith IL-2 and/or IL-3 genes decreased significantly inour privious report. In this study, recombinantadenoviruses were used to evaluate the therapeuticpotential of combined IL-2/IL-3 gene therapy in thetreatment of established subcutaneous tumor model ofG422 glioblastoma. Murine IL-2, IL-3 recombinantadenoviruses (2×10~8 pfu) were injected directly into
1997年03期 296-297页 [查看摘要][在线阅读][下载 104k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> The prognosis for patients with primarymalignant glioma remains poor, in spite of a varietyof different forms of treatments. Broad suppressionof humoral and cell-mediated immunity is found inpatients with malignant gliomas. Interleukin-2 (IL-2)production and IL-2 receptor expression are decreased
1997年03期 297页 [查看摘要][在线阅读][下载 51k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] <正> HSV-TK/GCV system and CD/5FC system aretwo effective systems of cancer suicide gene therapy.We have demonstrated that adenovirus-mediated CD/5FC system could suppress the growth of subcutaneoushuman hepatocellular carcinoma to some degree. Tofurther investigate whether the suicide gene therapyaffects the immune system or the immune systemaffects the effectiveness of suicide gene therapy, acolon adenocarcinoma of BALB/c mice, CT26, was
1997年03期 297-298页 [查看摘要][在线阅读][下载 107k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> The prognosis for patients with primarymalignant glioma remains poor, in spite of a varietyof different forms of treatments. Broad suppressionof humoral and cell-mediated immunity is found inpatients with malignant gliomas. Interleukin-2 (IL-2)production and IL-2 receptor expression are decreased
1997年03期 297页 [查看摘要][在线阅读][下载 51k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] <正> HSV-TK/GCV system and CD/5FC system aretwo effective systems of cancer suicide gene therapy.We have demonstrated that adenovirus-mediated CD/5FC system could suppress the growth of subcutaneoushuman hepatocellular carcinoma to some degree. Tofurther investigate whether the suicide gene therapyaffects the immune system or the immune systemaffects the effectiveness of suicide gene therapy, acolon adenocarcinoma of BALB/c mice, CT26, was
1997年03期 297-298页 [查看摘要][在线阅读][下载 107k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] - Hiroaki Wakimoto,Hirofumi Hamada
<正> Hepatocellular carcinoma (HCC) is one of the mostcommon cancers in the world, especially in East Asia.There is no standardized or effective strategy could beadapted routinely except of some early diagnosedpatients, and the prognosis is poor. In recent years, genetherapy has become a standard experimental approach fortreating cancers that have escaped conventionaltherapies. One such an approach is to confer the tumorcells with sensitivity to chemical reagents through genetic
1997年03期 298-299页 [查看摘要][在线阅读][下载 109k] [下载次数：12 ] |[引用频次：0 ] |[阅读次数：0 ] - Hiroaki Wakimoto,Hirofumi Hamada
<正> Hepatocellular carcinoma (HCC) is one of the mostcommon cancers in the world, especially in East Asia.There is no standardized or effective strategy could beadapted routinely except of some early diagnosedpatients, and the prognosis is poor. In recent years, genetherapy has become a standard experimental approach fortreating cancers that have escaped conventionaltherapies. One such an approach is to confer the tumorcells with sensitivity to chemical reagents through genetic
1997年03期 298-299页 [查看摘要][在线阅读][下载 109k] [下载次数：12 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Antitumor effects of combined transfer of suicidegene and cytokine gene were investigated in this report.Adenovirus harboring E.coli. cytosine deaminase (CD)gene (Ad-CD) and murine interleukin 2 (IL-2) gene(Ad-IL-2) were used for gene transfer in vitro and invivo. C57BL/6 mice were inoculated subcutaneously withB16F10 melanoma cells and three days later treated withadenovirus injection at the site of tumor inoculation.Significant inhibition of tumor growth was achieved after
1997年03期 299页 [查看摘要][在线阅读][下载 54k] [下载次数：7 ] |[引用频次：0 ] |[阅读次数：0 ] <正> A plasmid expressing antisense MDRl cDNAsegment was introduced into KB_(v200) which inductedmultiple drugs to VCT (vincristine, 175 fold-resistancehigher than that in original KB cells) and ADM(adriamycin, 14. 5 fold) resulting over-expression ofMDRl. We used the primers for antisense RNA asfollowing: upstream 5′OGAATTCTGAAACCTGTAAGCAGCAACC 3′: downstream 5′CGGGATCCTCGA
1997年03期 299-300页 [查看摘要][在线阅读][下载 116k] [下载次数：8 ] |[引用频次：1 ] |[阅读次数：0 ] -
<正> Antitumor effects of combined transfer of suicidegene and cytokine gene were investigated in this report.Adenovirus harboring E.coli. cytosine deaminase (CD)gene (Ad-CD) and murine interleukin 2 (IL-2) gene(Ad-IL-2) were used for gene transfer in vitro and invivo. C57BL/6 mice were inoculated subcutaneously withB16F10 melanoma cells and three days later treated withadenovirus injection at the site of tumor inoculation.Significant inhibition of tumor growth was achieved after
1997年03期 299页 [查看摘要][在线阅读][下载 54k] [下载次数：7 ] |[引用频次：0 ] |[阅读次数：0 ] <正> A plasmid expressing antisense MDRl cDNAsegment was introduced into KB_(v200) which inductedmultiple drugs to VCT (vincristine, 175 fold-resistancehigher than that in original KB cells) and ADM(adriamycin, 14. 5 fold) resulting over-expression ofMDRl. We used the primers for antisense RNA asfollowing: upstream 5′OGAATTCTGAAACCTGTAAGCAGCAACC 3′: downstream 5′CGGGATCCTCGA
1997年03期 299-300页 [查看摘要][在线阅读][下载 116k] [下载次数：8 ] |[引用频次：1 ] |[阅读次数：0 ] -
<正> It's well known that interleukin-2 (IL-2) plays animportant role in eliciting antitumor immunityparticularly mediated by T cells. In addition, theexpression of MHC can be enhanced by IL-2 genetransfection in tumor cells. Recent studies have indicatedthat at least two signals are required for the activation ofnaive T cells by antigen-bearing target cells: an antigen-sopific signal and a crystimulatory signal. B7-l is an
1997年03期 300页 [查看摘要][在线阅读][下载 63k] [下载次数：11 ] |[引用频次：0 ] |[阅读次数：0 ] - Jesus Prieto
<正> Background: Unresectable pancreatic cancer haspoor prognosis and no effective treatment. Somatic genetherapy is currently being investigated as a possibletreatment for malignancies. One of the most promisingstrategies is the introduction of herpes simplex virus type-1 thymidine kinase (HSV-tk) in the cells, followed bytreatment with the antiviral drug ganciclovir (GCV).
1997年03期 300-301页 [查看摘要][在线阅读][下载 117k] [下载次数：7 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> It's well known that interleukin-2 (IL-2) plays animportant role in eliciting antitumor immunityparticularly mediated by T cells. In addition, theexpression of MHC can be enhanced by IL-2 genetransfection in tumor cells. Recent studies have indicatedthat at least two signals are required for the activation ofnaive T cells by antigen-bearing target cells: an antigen-sopific signal and a crystimulatory signal. B7-l is an
1997年03期 300页 [查看摘要][在线阅读][下载 63k] [下载次数：11 ] |[引用频次：0 ] |[阅读次数：0 ] - Jesus Prieto
<正> Background: Unresectable pancreatic cancer haspoor prognosis and no effective treatment. Somatic genetherapy is currently being investigated as a possibletreatment for malignancies. One of the most promisingstrategies is the introduction of herpes simplex virus type-1 thymidine kinase (HSV-tk) in the cells, followed bytreatment with the antiviral drug ganciclovir (GCV).
1997年03期 300-301页 [查看摘要][在线阅读][下载 117k] [下载次数：7 ] |[引用频次：0 ] |[阅读次数：0 ] - Miguel Idoate,Roberto Bilbao,Bruno Sangro,Oscar Bruna,Jesus Vazquez,Jesus Prieto
<正> Viral-mediated gene transfer of thymidine kinase ofherpes simplex virus (HSV-tk) has been used to confercytotoxic sensitivity to ganciclovir (GCV) in a variety oftnmor cells. HSV-tk converts GCV into a phosphorylatedcompound which is toxic for dividing cells by blockingDNA synthesis. Our previous study has shown that
1997年03期 301-302页 [查看摘要][在线阅读][下载 111k] [下载次数：13 ] |[引用频次：4 ] |[阅读次数：0 ] - Miguel Idoate,Roberto Bilbao,Bruno Sangro,Oscar Bruna,Jesus Vazquez,Jesus Prieto
<正> Viral-mediated gene transfer of thymidine kinase ofherpes simplex virus (HSV-tk) has been used to confercytotoxic sensitivity to ganciclovir (GCV) in a variety oftnmor cells. HSV-tk converts GCV into a phosphorylatedcompound which is toxic for dividing cells by blockingDNA synthesis. Our previous study has shown that
1997年03期 301-302页 [查看摘要][在线阅读][下载 111k] [下载次数：13 ] |[引用频次：4 ] |[阅读次数：0 ] -
<正> With deep research into apoptosis recently,malignancy has been recognized to result not only fromenhanced cell proliferation but also from decreasedphysiological programmed cell death (apoptosis).Interleukin-l β converting enzyme (ICE) gene, ahomologue of the Caenorhabiditis elegans ce1l death geneced-3, has been identified as a key inducer of apoptosis inmammalian cells. However, no study of ICE genetherapy on cancer has been reported up to now. Weconstructed recombinant retroviral vectors with the
1997年03期 302页 [查看摘要][在线阅读][下载 56k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Heat shock protein 90 (Hsp90) is a major heatshock protein whose functions are necessary for livingorganisms, including both procaryotes and eucaryotes.Human Hsp90 consists of two forms, Hsp90α andHsp90β. Expression of Hsp90α appears to be inducible bydifferent stimuli. Adenovirus EIA gene products andtransformation with the H-ras oncogene can induce aselective overexpression of Hsp90α. Hsp90α was found
1997年03期 302-303页 [查看摘要][在线阅读][下载 112k] [下载次数：13 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> With deep research into apoptosis recently,malignancy has been recognized to result not only fromenhanced cell proliferation but also from decreasedphysiological programmed cell death (apoptosis).Interleukin-l β converting enzyme (ICE) gene, ahomologue of the Caenorhabiditis elegans ce1l death geneced-3, has been identified as a key inducer of apoptosis inmammalian cells. However, no study of ICE genetherapy on cancer has been reported up to now. Weconstructed recombinant retroviral vectors with the
1997年03期 302页 [查看摘要][在线阅读][下载 56k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Heat shock protein 90 (Hsp90) is a major heatshock protein whose functions are necessary for livingorganisms, including both procaryotes and eucaryotes.Human Hsp90 consists of two forms, Hsp90α andHsp90β. Expression of Hsp90α appears to be inducible bydifferent stimuli. Adenovirus EIA gene products andtransformation with the H-ras oncogene can induce aselective overexpression of Hsp90α. Hsp90α was found
1997年03期 302-303页 [查看摘要][在线阅读][下载 112k] [下载次数：13 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Interleukin-15 (IL-15) is a novel cytokine thatstimulates the proliferation of T cells, B cells and NKcells. Several biological activities of interleukin-15overlap those of IL-2. Here, we report that lung cancercells. which were modified by IL-15 cDNA, may serve astumor vaccine that plays an antitumor role in murinemodel. IL-15 cDNA was amplified by RT-PCR withmRNA templates that were extracted from human
1997年03期 303-304页 [查看摘要][在线阅读][下载 112k] [下载次数：7 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Interleukin-15 (IL-15) is a novel cytokine thatstimulates the proliferation of T cells, B cells and NKcells. Several biological activities of interleukin-15overlap those of IL-2. Here, we report that lung cancercells. which were modified by IL-15 cDNA, may serve astumor vaccine that plays an antitumor role in murinemodel. IL-15 cDNA was amplified by RT-PCR withmRNA templates that were extracted from human
1997年03期 303-304页 [查看摘要][在线阅读][下载 112k] [下载次数：7 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> To apply gene therapy to breast carcinoma wepropose a model for in vitro gene transfer of the herpessimplex thymidine kinase (HSV-tk) gene using Stkvector-producing cells. In this study the HSV-tk gene wastransferred to mouse mammary carcinoma cell line 4Tlwith the method of infection. The HSV-tk gene in thegenome of transduced cells (4Tl/tk) was demonstratedby the method of DNA-PCR. Using a modified XTT
1997年03期 304页 [查看摘要][在线阅读][下载 57k] [下载次数：14 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Anti-Fas or Fas ligand can induce apoptosis whenthey bind Fas antigen on cell surfaces, and have potentialtherapeutic uses. But the disadvantage is obvious asnormal cells can also be killed. In this paper, weconstructed a fusion protein between the transmembraneand cytoplasmic domains of hfas and the HBD (hormonebinding domain) of hER (referred to as MfasER).Glioma cells transfeced with MfasER could be indued to
1997年03期 304-305页 [查看摘要][在线阅读][下载 111k] [下载次数：8 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> To apply gene therapy to breast carcinoma wepropose a model for in vitro gene transfer of the herpessimplex thymidine kinase (HSV-tk) gene using Stkvector-producing cells. In this study the HSV-tk gene wastransferred to mouse mammary carcinoma cell line 4Tlwith the method of infection. The HSV-tk gene in thegenome of transduced cells (4Tl/tk) was demonstratedby the method of DNA-PCR. Using a modified XTT
1997年03期 304页 [查看摘要][在线阅读][下载 57k] [下载次数：14 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Anti-Fas or Fas ligand can induce apoptosis whenthey bind Fas antigen on cell surfaces, and have potentialtherapeutic uses. But the disadvantage is obvious asnormal cells can also be killed. In this paper, weconstructed a fusion protein between the transmembraneand cytoplasmic domains of hfas and the HBD (hormonebinding domain) of hER (referred to as MfasER).Glioma cells transfeced with MfasER could be indued to
1997年03期 304-305页 [查看摘要][在线阅读][下载 111k] [下载次数：8 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Tumor cells may escape immune surveillance mainlyby (1) down regulation of major histocompatibilitycomplex (MHC) molecules or alteration of antigen-processing pathways; (2) low-level of cytokines in thevicinity of the tumor, which can't activate immunosystemeffectively. Recent studies have shown that geneticallyengineered tumor cells expressing cytokines such as IL-2IFN-γ can induce greater CTL activity and activate NK,LAK and TIL. Besides IFN-γ can enhance the expression
1997年03期 305页 [查看摘要][在线阅读][下载 55k] [下载次数：8 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Tumor necrosis factor α (TNFα) and interleukin 2(IL-2) are cytokines that possess potent antitumor andimmunomodulatory activity such as enhancing monocyte-macrophage and neutrophil cytotoxic activities,increasing T cell proliferation and IL-2 receptorexpression and augmenting cytotoxicity of cytotoxic Tlymphocyte. To investigate the therapeutic effect of thedirect cytokine gene transfer, recombinant adenovirus
1997年03期 305-306页 [查看摘要][在线阅读][下载 111k] [下载次数：8 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Tumor cells may escape immune surveillance mainlyby (1) down regulation of major histocompatibilitycomplex (MHC) molecules or alteration of antigen-processing pathways; (2) low-level of cytokines in thevicinity of the tumor, which can't activate immunosystemeffectively. Recent studies have shown that geneticallyengineered tumor cells expressing cytokines such as IL-2IFN-γ can induce greater CTL activity and activate NK,LAK and TIL. Besides IFN-γ can enhance the expression
1997年03期 305页 [查看摘要][在线阅读][下载 55k] [下载次数：8 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Tumor necrosis factor α (TNFα) and interleukin 2(IL-2) are cytokines that possess potent antitumor andimmunomodulatory activity such as enhancing monocyte-macrophage and neutrophil cytotoxic activities,increasing T cell proliferation and IL-2 receptorexpression and augmenting cytotoxicity of cytotoxic Tlymphocyte. To investigate the therapeutic effect of thedirect cytokine gene transfer, recombinant adenovirus
1997年03期 305-306页 [查看摘要][在线阅读][下载 111k] [下载次数：8 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> The whole coding region including signal peptidesequence of mouse interleukin-2 (mIL-2) cDNA wasobtained by PCR method and then cloned into the propersites of pUC18 vector. Sequencing analysis throughSanger method (dideoxy-mediated chain-terminationmethod) proved the consistency of mIL-2 cDNAsequence with that reported before. The fragment was
1997年03期 306页 [查看摘要][在线阅读][下载 56k] [下载次数：12 ] |[引用频次：0 ] |[阅读次数：0 ] <正> In order to investigate the effects of cytokine fusedgene transfection on tumor cell modification, threeretroviral vectors for human interleukin 6 (IL-6),interleukin 2 (IL-2) and, IL-6/IL-2 fusion gene wereconstructed. The retroviral vectors were introduced intomurine B16 melanoma cell line respectively and theeffects of single or fused cytokine gene on tumor cellbiology in vitro and in vivo were examined. In vivo, thethree cytokine gene-modified B16 cells all showeddecreased tumorigenicity and metastatic potentials,however, the reduction in tumorigenicity and metastasis
1997年03期 306-307页 [查看摘要][在线阅读][下载 109k] [下载次数：14 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> The whole coding region including signal peptidesequence of mouse interleukin-2 (mIL-2) cDNA wasobtained by PCR method and then cloned into the propersites of pUC18 vector. Sequencing analysis throughSanger method (dideoxy-mediated chain-terminationmethod) proved the consistency of mIL-2 cDNAsequence with that reported before. The fragment was
1997年03期 306页 [查看摘要][在线阅读][下载 56k] [下载次数：12 ] |[引用频次：0 ] |[阅读次数：0 ] <正> In order to investigate the effects of cytokine fusedgene transfection on tumor cell modification, threeretroviral vectors for human interleukin 6 (IL-6),interleukin 2 (IL-2) and, IL-6/IL-2 fusion gene wereconstructed. The retroviral vectors were introduced intomurine B16 melanoma cell line respectively and theeffects of single or fused cytokine gene on tumor cellbiology in vitro and in vivo were examined. In vivo, thethree cytokine gene-modified B16 cells all showeddecreased tumorigenicity and metastatic potentials,however, the reduction in tumorigenicity and metastasis
1997年03期 306-307页 [查看摘要][在线阅读][下载 109k] [下载次数：14 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> In order to investigate the possible relationshipbetween the transfection of IL-2 gene and the changes intumor cells biological characteristics, we used retroviralvector to transduce human mammary carcinoma MCF-7cell line with the IL-2 cDNA and explored the changes incell membrane components and function. The genemodified cells releasing 70 - 169 U/106 cells of IL-2 in 24hrs. showed apparent morphological conversion includingthe appearance change from epitheloid form to
1997年03期 307页 [查看摘要][在线阅读][下载 53k] [下载次数：8 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Patients with malignant glioma have a poorprognosis despite the combined use of surgery,irradiation, chemotherapy, and a variety ofimmunotherapies. These patient are known to showdecreased circulatory immune function and suppression ofthe tumor-specific immune response. Interferon-γ(IFN-
1997年03期 307-308页 [查看摘要][在线阅读][下载 109k] [下载次数：8 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> In order to investigate the possible relationshipbetween the transfection of IL-2 gene and the changes intumor cells biological characteristics, we used retroviralvector to transduce human mammary carcinoma MCF-7cell line with the IL-2 cDNA and explored the changes incell membrane components and function. The genemodified cells releasing 70 - 169 U/106 cells of IL-2 in 24hrs. showed apparent morphological conversion includingthe appearance change from epitheloid form to
1997年03期 307页 [查看摘要][在线阅读][下载 53k] [下载次数：8 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Patients with malignant glioma have a poorprognosis despite the combined use of surgery,irradiation, chemotherapy, and a variety ofimmunotherapies. These patient are known to showdecreased circulatory immune function and suppression ofthe tumor-specific immune response. Interferon-γ(IFN-
1997年03期 307-308页 [查看摘要][在线阅读][下载 109k] [下载次数：8 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> To study the effects of human interleukin-2 genetransfer on the morphology, proliferation, tumori-genesis, immunity, and oncogenes expression of tumorcells, the gene encoding human interleukin-2 (IL-2) wasintroduced via retroviral vectors pLXSN into ovariancancer cell lines SKOV3. A resistant clone with high IL-2activity (318 U/10~6cell/24 hours) in the culturesupernatants was cloned. The hIL-2 mRNA was detectedwith RT-PCR in SKOV3/IL2 cells. The IL2 secretinghas persisted for 23 weeks with 38 passanges. The hIL-2
1997年03期 308页 [查看摘要][在线阅读][下载 56k] [下载次数：11 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Glioblastoma multiforme (GBM), the most commonprimary malignant brain tumor in adults is associated witha poor prognosis despite aggressive treatment withsurgical tumor debulking, radiation, and chemotherapy.Novel approaches including gene therapy provide newalternatives in the treatment of GBM. Apo-2 ligand(Apo-2L), a novel cytokine, is a member of the tumor
1997年03期 308-309页 [查看摘要][在线阅读][下载 113k] [下载次数：11 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> To study the effects of human interleukin-2 genetransfer on the morphology, proliferation, tumori-genesis, immunity, and oncogenes expression of tumorcells, the gene encoding human interleukin-2 (IL-2) wasintroduced via retroviral vectors pLXSN into ovariancancer cell lines SKOV3. A resistant clone with high IL-2activity (318 U/10~6cell/24 hours) in the culturesupernatants was cloned. The hIL-2 mRNA was detectedwith RT-PCR in SKOV3/IL2 cells. The IL2 secretinghas persisted for 23 weeks with 38 passanges. The hIL-2
1997年03期 308页 [查看摘要][在线阅读][下载 56k] [下载次数：11 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Glioblastoma multiforme (GBM), the most commonprimary malignant brain tumor in adults is associated witha poor prognosis despite aggressive treatment withsurgical tumor debulking, radiation, and chemotherapy.Novel approaches including gene therapy provide newalternatives in the treatment of GBM. Apo-2 ligand(Apo-2L), a novel cytokine, is a member of the tumor
1997年03期 308-309页 [查看摘要][在线阅读][下载 113k] [下载次数：11 ] |[引用频次：0 ] |[阅读次数：0 ] - Hirofumi Hamada
<正> Dendritic cells (DC) play a critical role in theinduction of immune responses, and are utilized as potentadjuvants in cancer immunotherapy. Several strategies forloading DC with tumor antigens have been developed.Vaccination with tumor antigen-loaded DC wasdemonstrated to be capable of inducing antitumorimmunity, but some limitations still exist. So it's
1997年03期 309-310页 [查看摘要][在线阅读][下载 115k] [下载次数：16 ] |[引用频次：0 ] |[阅读次数：0 ] - Hirofumi Hamada
<正> Dendritic cells (DC) play a critical role in theinduction of immune responses, and are utilized as potentadjuvants in cancer immunotherapy. Several strategies forloading DC with tumor antigens have been developed.Vaccination with tumor antigen-loaded DC wasdemonstrated to be capable of inducing antitumorimmunity, but some limitations still exist. So it's
1997年03期 309-310页 [查看摘要][在线阅读][下载 115k] [下载次数：16 ] |[引用频次：0 ] |[阅读次数：0 ] - Sophie Cayeux,Günther Richter,Gabrielle Noffz,Bernd Drken,Thomas Blankenstein
<正> We have previously shown that tumor cellsgenetically modified to co-express IL-4 or IL-7 and B7. 1have increased immunogenicity and provided vaccinessuperior to single gene transfectants or tumor cells/adjuvant mixture. Tumor antigens can be presented eitherdirectly by tumor cells or be indirectly represented byhost APC (cross-priming). Here we asked whether B7. 1and IL-7 or IL-4 improved in an additive fashion one
1997年03期 310页 [查看摘要][在线阅读][下载 58k] [下载次数：13 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Severe combined immunodeficient (SCID) mice lackfunctional B and T lymphocytes which make themamicable recipient for xenograft transplantation. Amplestudies have demonstrated uptake of human tumors andengrafment of human lymphocytes in the scid/scid mice.A human-lymphocyte-chimera-SCID mouse bearinghuman tumor is a model mimicking human cancer patientand can be valuable for studying immunogene therapeuticregimens on cancer. However, the existing NK functionand the leakiness of the scid/scid mouse strain greatlydiminish the engraftment rate of human tumors or humanimmune cells (usually around 60%). Here wedemonstrate using the scid/beige strain, which lacksfunctional B, T, and NK cells, significantly improvedrate of tumor uptake and human lymphocytereconstitution. A variety of unmodified or immuno-modulatory gene-transduced human tumor cells includingmelanoma, hepatoma, glioblastoma, and breast cancercells were successfully established in the scid/beige mice.Palpable tumors were usually conceived at 1 -
1997年03期 310-311页 [查看摘要][在线阅读][下载 111k] [下载次数：15 ] |[引用频次：0 ] |[阅读次数：0 ] - Sophie Cayeux,Günther Richter,Gabrielle Noffz,Bernd Drken,Thomas Blankenstein
<正> We have previously shown that tumor cellsgenetically modified to co-express IL-4 or IL-7 and B7. 1have increased immunogenicity and provided vaccinessuperior to single gene transfectants or tumor cells/adjuvant mixture. Tumor antigens can be presented eitherdirectly by tumor cells or be indirectly represented byhost APC (cross-priming). Here we asked whether B7. 1and IL-7 or IL-4 improved in an additive fashion one
1997年03期 310页 [查看摘要][在线阅读][下载 58k] [下载次数：13 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Severe combined immunodeficient (SCID) mice lackfunctional B and T lymphocytes which make themamicable recipient for xenograft transplantation. Amplestudies have demonstrated uptake of human tumors andengrafment of human lymphocytes in the scid/scid mice.A human-lymphocyte-chimera-SCID mouse bearinghuman tumor is a model mimicking human cancer patientand can be valuable for studying immunogene therapeuticregimens on cancer. However, the existing NK functionand the leakiness of the scid/scid mouse strain greatlydiminish the engraftment rate of human tumors or humanimmune cells (usually around 60%). Here wedemonstrate using the scid/beige strain, which lacksfunctional B, T, and NK cells, significantly improvedrate of tumor uptake and human lymphocytereconstitution. A variety of unmodified or immuno-modulatory gene-transduced human tumor cells includingmelanoma, hepatoma, glioblastoma, and breast cancercells were successfully established in the scid/beige mice.Palpable tumors were usually conceived at 1 -
1997年03期 310-311页 [查看摘要][在线阅读][下载 111k] [下载次数：15 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Since the beginning of gene therapy, most of genetransfection were focused on the tumor cells or effectorcells. We selected macrophages as the target cells of genetransfection because they are not only antitumor effectorcells but also antigen-presenting cells.They act as abridge connecting tumor cells with immune effector cells.Two cytokines we chosen are closely linkcd with thefunctions of macrophage. IFN-γis a principle factor toactivate macrophages and it incrcases MHC expression ofthem which can improve their antigen presenting ability.M-CSF is an important cytokine to keep theproliferation, differentiation and maturation ofmacrophage progenitor cells. In this study, we used a
1997年03期 311-312页 [查看摘要][在线阅读][下载 111k] [下载次数：8 ] |[引用频次：0 ] |[阅读次数：0 ] <正> The ultimate aim of cancer immunotherapy is theinduction of tumor-specific T-lymphocyte responseseffective in eradicating disseminated tumors. There aregenerally tumor infiltrating T lymphocytes in situ oftumor, but most of which are inactive, one of the majorreasons of such Inactivation is that T lymphocytes areexposured to an antigen-specific TCR signal in theabsence of antigen-nonspecific or co-stimulatory signal.The co-stimulatory factors, B7-l and B7-2 on the surfaceof antigen presenting cells provide a key signal for thegeneration of T cell immunity. Sincc hepatoma usually
1997年03期 311页 [查看摘要][在线阅读][下载 54k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Since the beginning of gene therapy, most of genetransfection were focused on the tumor cells or effectorcells. We selected macrophages as the target cells of genetransfection because they are not only antitumor effectorcells but also antigen-presenting cells.They act as abridge connecting tumor cells with immune effector cells.Two cytokines we chosen are closely linkcd with thefunctions of macrophage. IFN-γis a principle factor toactivate macrophages and it incrcases MHC expression ofthem which can improve their antigen presenting ability.M-CSF is an important cytokine to keep theproliferation, differentiation and maturation ofmacrophage progenitor cells. In this study, we used a
1997年03期 311-312页 [查看摘要][在线阅读][下载 111k] [下载次数：8 ] |[引用频次：0 ] |[阅读次数：0 ] <正> The ultimate aim of cancer immunotherapy is theinduction of tumor-specific T-lymphocyte responseseffective in eradicating disseminated tumors. There aregenerally tumor infiltrating T lymphocytes in situ oftumor, but most of which are inactive, one of the majorreasons of such Inactivation is that T lymphocytes areexposured to an antigen-specific TCR signal in theabsence of antigen-nonspecific or co-stimulatory signal.The co-stimulatory factors, B7-l and B7-2 on the surfaceof antigen presenting cells provide a key signal for thegeneration of T cell immunity. Sincc hepatoma usually
1997年03期 311页 [查看摘要][在线阅读][下载 54k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> The murine interleukin-2 gene was introduced intomurine melanoma B16 cells by retroviral vector. AfterG418 selection, a clone stably secreting high level ofinterleukin-2 (1038IU per 10~6 cells per 24 hours) wasobtained. The tumorigenicity of this clone decreasedsignificantly as compared with parental tumor cells andtumor cells transferred neomycin gene. There was notumor developed after injection of 5×10~5 IL2-transfectedB16 cells into C57BL/6 mice. However when the amount
1997年03期 312-313页 [查看摘要][在线阅读][下载 113k] [下载次数：12 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Induction of tumor-specific cellular immune responseis very important in the cancer therapy. In this study,we used tumor antigen obtained by thaw of melanomacells to pulse M-CSF or/and IFN-γ gene-modificdmacrophages before in viro infusion. Tumor membraneantigens could be phagocytosed by macrophages inculture. Antigen processing and mcxlulation of thepresentation can be achieved before macrophageinjection. The tumor antigens will be processedintracellularly by macrophages and thereafter presented
1997年03期 312页 [查看摘要][在线阅读][下载 57k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> The murine interleukin-2 gene was introduced intomurine melanoma B16 cells by retroviral vector. AfterG418 selection, a clone stably secreting high level ofinterleukin-2 (1038IU per 10~6 cells per 24 hours) wasobtained. The tumorigenicity of this clone decreasedsignificantly as compared with parental tumor cells andtumor cells transferred neomycin gene. There was notumor developed after injection of 5×10~5 IL2-transfectedB16 cells into C57BL/6 mice. However when the amount
1997年03期 312-313页 [查看摘要][在线阅读][下载 113k] [下载次数：12 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Induction of tumor-specific cellular immune responseis very important in the cancer therapy. In this study,we used tumor antigen obtained by thaw of melanomacells to pulse M-CSF or/and IFN-γ gene-modificdmacrophages before in viro infusion. Tumor membraneantigens could be phagocytosed by macrophages inculture. Antigen processing and mcxlulation of thepresentation can be achieved before macrophageinjection. The tumor antigens will be processedintracellularly by macrophages and thereafter presented
1997年03期 312页 [查看摘要][在线阅读][下载 57k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> The progressive growth of tumors in the presence ofan intact immune system suggests that immunemechanisms may have failed to defend the host fromtumor cells. One therapeutic approach to cnhanceeffective immune responses is to introduce the immuno-regulatory genes that alter the local immunologicalmicroenvironment and increase immunogenecity of tumor
1997年03期 313-314页 [查看摘要][在线阅读][下载 111k] [下载次数：15 ] |[引用频次：0 ] |[阅读次数：0 ] <正> The progressive growth of tumors in the presence ofan intact immune system suggests that immunemechanisms may have failed to defend the host fromtumor cells. One therapeutic approach to cnhanceeffective immune responses is to introduce the immuno-regulatory genes that alter the local immunologicalmicroenvironment and increase immunogenecity of tumor
1997年03期 313-314页 [查看摘要][在线阅读][下载 111k] [下载次数：15 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Over the past 10 years adoptive immunotherapieshave been developed for cancer treatment. Cytotoxic Tlymphocytes (CTL) play a major role in host antitumorimmune response. The perforin and Fas ligand (Fas-L)pathways which were two major mechanisms are res-ponsible for tumor cell death by CTLs. A major obstacleto the application of adoptive imunotherapy in thetreatment of human malignancy has been the inability to
1997年03期 314页 [查看摘要][在线阅读][下载 56k] [下载次数：11 ] |[引用频次：0 ] |[阅读次数：0 ] <正>O—bjective:T——o study many aspects of humaninterferon-gamma (hulFN一7) gene transducedhepatocellular carcinoma cells(Hcc),including the up-
1997年03期 314-315页 [查看摘要][在线阅读][下载 110k] [下载次数：7 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Over the past 10 years adoptive immunotherapieshave been developed for cancer treatment. Cytotoxic Tlymphocytes (CTL) play a major role in host antitumorimmune response. The perforin and Fas ligand (Fas-L)pathways which were two major mechanisms are res-ponsible for tumor cell death by CTLs. A major obstacleto the application of adoptive imunotherapy in thetreatment of human malignancy has been the inability to
1997年03期 314页 [查看摘要][在线阅读][下载 56k] [下载次数：11 ] |[引用频次：0 ] |[阅读次数：0 ] <正>O—bjective:T——o study many aspects of humaninterferon-gamma (hulFN一7) gene transducedhepatocellular carcinoma cells(Hcc),including the up-
1997年03期 314-315页 [查看摘要][在线阅读][下载 110k] [下载次数：7 ] |[引用频次：0 ] |[阅读次数：0 ] - Gabriele Noffz,Mariette Mohaupt,Thomas Blankenstein
<正> A wide variety of human tumors express interleukin10 (IL-10) for reasons poorly understood. We haveanalysed the effect of spontaneous IL-10 expression by amouse tumor (J558L) on its immunparalysing effect.Because cross-priming" of T cells by host antigenpresenting cells for MHC class I restricted tumor antigensis a major pathway for induction of tumor immunity andthat is enhanced by granulocyte-macrophage colony-stimulating factor (GM-CSF), we expressed this cytokinein J558L cells. GM-CSF secreting cells were not effective
1997年03期 315页 [查看摘要][在线阅读][下载 55k] [下载次数：12 ] |[引用频次：1 ] |[阅读次数：0 ] - Gabriele Noffz,Mariette Mohaupt,Thomas Blankenstein
<正> A wide variety of human tumors express interleukin10 (IL-10) for reasons poorly understood. We haveanalysed the effect of spontaneous IL-10 expression by amouse tumor (J558L) on its immunparalysing effect.Because cross-priming" of T cells by host antigenpresenting cells for MHC class I restricted tumor antigensis a major pathway for induction of tumor immunity andthat is enhanced by granulocyte-macrophage colony-stimulating factor (GM-CSF), we expressed this cytokinein J558L cells. GM-CSF secreting cells were not effective
1997年03期 315页 [查看摘要][在线阅读][下载 55k] [下载次数：12 ] |[引用频次：1 ] |[阅读次数：0 ] - G.Richter,S.Cayeux,C.Becker,C.Beck,A.Aicher,B.Drken,T.Blankenstein
<正> Gene modification of tumor cells to express a varietyof cytokines such as IL-2 or the co-stimulatory moleculeB7. 1 led to increased immunogenicity and reducedtumorigenicity of tumors has several models and thisprocess involves T cells. We have previously reporteddecreased tumorigenicity of the murine plasmacytomaJ558L (MHC class Ⅰ~+ and class Ⅱ~-) expressing IL-2 orB7. l. When systemic immunity was analyzed,
1997年03期 315-316页 [查看摘要][在线阅读][下载 110k] [下载次数：7 ] |[引用频次：0 ] |[阅读次数：0 ] - G.Richter,S.Cayeux,C.Becker,C.Beck,A.Aicher,B.Drken,T.Blankenstein
<正> Gene modification of tumor cells to express a varietyof cytokines such as IL-2 or the co-stimulatory moleculeB7. 1 led to increased immunogenicity and reducedtumorigenicity of tumors has several models and thisprocess involves T cells. We have previously reporteddecreased tumorigenicity of the murine plasmacytomaJ558L (MHC class Ⅰ~+ and class Ⅱ~-) expressing IL-2 orB7. l. When systemic immunity was analyzed,
1997年03期 315-316页 [查看摘要][在线阅读][下载 110k] [下载次数：7 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Recent studies suggest that MHC class Ⅱ genetransfer could stimulate protective antitumor immunity.Whether xenogeneic MHC Ⅱ transfected tumor cells havethese effect is unknown. In this study, HLA-DR3 α and βchain cDNA were cloned by RT-PCR from human Blymphoma Raji cell line and confirmed by DNAsequencing. The recombinant expressing vectors wereconstructed by inserting the α and β chain cDNA intoPCEP4/pLXSN vectors respectively. After transfectingby lipofectamine and selecting with G418 and Hyg, theHLA-DR3 transfected mouse melanoma B16 recombinantclones were obtained. Approximately 59% of the
1997年03期 316页 [查看摘要][在线阅读][下载 56k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Recombinant vaccinia virus has many advantagesover more restricted vectors like retrovirus andadenovirus. The proven safety of vaccinia virus, which isrestricted to local and transitory infection, favors clinicalapplication of vaccinia virus to deliver cytokines locally.
1997年03期 316-317页 [查看摘要][在线阅读][下载 110k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Recent studies suggest that MHC class Ⅱ genetransfer could stimulate protective antitumor immunity.Whether xenogeneic MHC Ⅱ transfected tumor cells havethese effect is unknown. In this study, HLA-DR3 α and βchain cDNA were cloned by RT-PCR from human Blymphoma Raji cell line and confirmed by DNAsequencing. The recombinant expressing vectors wereconstructed by inserting the α and β chain cDNA intoPCEP4/pLXSN vectors respectively. After transfectingby lipofectamine and selecting with G418 and Hyg, theHLA-DR3 transfected mouse melanoma B16 recombinantclones were obtained. Approximately 59% of the
1997年03期 316页 [查看摘要][在线阅读][下载 56k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Recombinant vaccinia virus has many advantagesover more restricted vectors like retrovirus andadenovirus. The proven safety of vaccinia virus, which isrestricted to local and transitory infection, favors clinicalapplication of vaccinia virus to deliver cytokines locally.
1997年03期 316-317页 [查看摘要][在线阅读][下载 110k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] - Bruce Acres
<正> Oncolysate, a debris of tumor cells, has been provento be effective in tumor active immunotherapy, it wasreported that the vaccinia virus, especially recombinantvaccinia viruses encoding human IL-2 (rVV-IL-2 ),enhanced the immunogenicity of transfected tumor cells.In this experiment, the murine melanoma cell B16-F10oncolysates trans fected by rVV-IL-2 (IL-2VBO) wereused as vaccine. The IL-2VBO or TK-VBO was preparedby incubating B16-F10 cells with rVV-IL-2 or rVV-TK at
1997年03期 317页 [查看摘要][在线阅读][下载 54k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] - Bruce Acres
<正> Direct gene transfer into somatic tissue in vivo is adeveloping technology with potential application forcancer gene therapy. Retrovirus vector, which was aneffective vehicle, still has some disadvantages ingenerating high titer recombinant vectors andmanipulating to mediate in viro gene transfer. In thispaper, recombinant vaccinia virus vector encoding human
1997年03期 317-318页 [查看摘要][在线阅读][下载 113k] [下载次数：14 ] |[引用频次：0 ] |[阅读次数：0 ] - Bruce Acres
<正> Oncolysate, a debris of tumor cells, has been provento be effective in tumor active immunotherapy, it wasreported that the vaccinia virus, especially recombinantvaccinia viruses encoding human IL-2 (rVV-IL-2 ),enhanced the immunogenicity of transfected tumor cells.In this experiment, the murine melanoma cell B16-F10oncolysates trans fected by rVV-IL-2 (IL-2VBO) wereused as vaccine. The IL-2VBO or TK-VBO was preparedby incubating B16-F10 cells with rVV-IL-2 or rVV-TK at
1997年03期 317页 [查看摘要][在线阅读][下载 54k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] - Bruce Acres
<正> Direct gene transfer into somatic tissue in vivo is adeveloping technology with potential application forcancer gene therapy. Retrovirus vector, which was aneffective vehicle, still has some disadvantages ingenerating high titer recombinant vectors andmanipulating to mediate in viro gene transfer. In thispaper, recombinant vaccinia virus vector encoding human
1997年03期 317-318页 [查看摘要][在线阅读][下载 113k] [下载次数：14 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> The moditication of tumor cells or effcor cellsusing cytokine genes as a strategy to enhance hostantitumor immunity has been studied intensively over the
1997年03期 318-319页 [查看摘要][在线阅读][下载 115k] [下载次数：27 ] |[引用频次：0 ] |[阅读次数：0 ] <正> The moditication of tumor cells or effcor cellsusing cytokine genes as a strategy to enhance hostantitumor immunity has been studied intensively over the
1997年03期 318-319页 [查看摘要][在线阅读][下载 115k] [下载次数：27 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Objectives: To construct retroviral vectorsexpressing antisense polymeric RNAs targeted at the coresequence (+13-+47) of HIV-l TAR RNA, and to testthe anti-HIV properties of this construct in transducedCD4~+T cells. Methods: The polymeric TAR-Core DNAwas amplified by the"self-primers-template PCR"
1997年03期 319-320页 [查看摘要][在线阅读][下载 112k] [下载次数：13 ] |[引用频次：0 ] |[阅读次数：0 ] <正> It has been demonstrated that tbe critical role ofbone marrow stromal cells (HMSCs ) is to sustain theselfrenewal of pluripotent hematopoietic stem cells andmaintain the homeostasis of bone marrow hematopoiesismicroenvironment. BMSC progenitor can differentiateinto several clements including macrophages, endothelialcells, fibroblasts and some other cells. Almost all
1997年03期 319页 [查看摘要][在线阅读][下载 56k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Objectives: To construct retroviral vectorsexpressing antisense polymeric RNAs targeted at the coresequence (+13-+47) of HIV-l TAR RNA, and to testthe anti-HIV properties of this construct in transducedCD4~+T cells. Methods: The polymeric TAR-Core DNAwas amplified by the"self-primers-template PCR"
1997年03期 319-320页 [查看摘要][在线阅读][下载 112k] [下载次数：13 ] |[引用频次：0 ] |[阅读次数：0 ] <正> It has been demonstrated that tbe critical role ofbone marrow stromal cells (HMSCs ) is to sustain theselfrenewal of pluripotent hematopoietic stem cells andmaintain the homeostasis of bone marrow hematopoiesismicroenvironment. BMSC progenitor can differentiateinto several clements including macrophages, endothelialcells, fibroblasts and some other cells. Almost all
1997年03期 319页 [查看摘要][在线阅读][下载 56k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Objective: To evaluate the efficiency and effect ofgene therapy for rabbit hind limb ischemia byintramuscular injection of VEGF cDNA. Methods:VEGF cDNA was obtained from the RNA in fetal liverwith RT-PCR and inserted into eukaryotic expressionvector, pSVK3, to generate pSV-VEGF. Thetranscription and expression of VEGF cDNA were
1997年03期 320页 [查看摘要][在线阅读][下载 56k] [下载次数：12 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Objective: The objective of research is to explorethe prospective for direct gene transfer to be applied as aform of gene therapy. Materials and methods: ①Experimental animals: Male BALB/c mice, 0 - 8 weeksold;②Preparations of plasmid: PCI-hIL-2 plasmid isexpression vector for human IL-2 containing cytome-galovirus immediate-early enhance/promoter region andT7 promoer;③Injection of plasmids: Male BALB/c
1997年03期 320-321页 [查看摘要][在线阅读][下载 113k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Objective: To evaluate the efficiency and effect ofgene therapy for rabbit hind limb ischemia byintramuscular injection of VEGF cDNA. Methods:VEGF cDNA was obtained from the RNA in fetal liverwith RT-PCR and inserted into eukaryotic expressionvector, pSVK3, to generate pSV-VEGF. Thetranscription and expression of VEGF cDNA were
1997年03期 320页 [查看摘要][在线阅读][下载 56k] [下载次数：12 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Objective: The objective of research is to explorethe prospective for direct gene transfer to be applied as aform of gene therapy. Materials and methods: ①Experimental animals: Male BALB/c mice, 0 - 8 weeksold;②Preparations of plasmid: PCI-hIL-2 plasmid isexpression vector for human IL-2 containing cytome-galovirus immediate-early enhance/promoter region andT7 promoer;③Injection of plasmids: Male BALB/c
1997年03期 320-321页 [查看摘要][在线阅读][下载 113k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Neurotrophic factors are able to promote neuronalsurvival and prevent neuronal death induced by variousinjuries and have potential uses in the treatment ofneuronal degenerative diseases and neuropathy. Due tothe existence of brain blood barrier, neurotrophic factorscan not be ased in CNS diseases directly. Gene therapy isa good candidate to overcome this problem. Since
1997年03期 321-322页 [查看摘要][在线阅读][下载 112k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Neurotrophic factors are able to promote neuronalsurvival and prevent neuronal death induced by variousinjuries and have potential uses in the treatment ofneuronal degenerative diseases and neuropathy. Due tothe existence of brain blood barrier, neurotrophic factorscan not be ased in CNS diseases directly. Gene therapy isa good candidate to overcome this problem. Since
1997年03期 321-322页 [查看摘要][在线阅读][下载 112k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] - Jennifer L.Horton,Elena B.Samoilova,Thomas A.Judge,Laurence A.Turka,James M.Wilson
<正> Both rheumatoid arthritis and animal models ofautoimmune arthritis are characterized by hyper-activation of synovial cells and hyperplasia of the synovialmembrane. The activated synovial cells produceinflammatory cytokines and degradative enzymes whichlead to destruction of cartilage and bones. Effectivetreatment of arthritis may require elimination of most orall activated synovial cells. The death factor Fas/Apo-1
1997年03期 322页 [查看摘要][在线阅读][下载 55k] [下载次数：11 ] |[引用频次：1 ] |[阅读次数：0 ] <正> Effects of fibroblast-mediated IL-2 and IL-3 genetherapy on recovery of bone marrow depression in tumor-bearing mice treated with cyclophosphamide (300 mg/kg) and syngeneic BMT were investigated in the presentreport. BALB/c mice were inoculated with J558Lplasmacytoma cells s. c. and 3 days later IL-2 secretingNIH3T3-IL-2, IL-3 secreting NIH3T3-IL-3 fibroblastcells were implanted into the tumor-bearing mice after
1997年03期 322-323页 [查看摘要][在线阅读][下载 108k] [下载次数：11 ] |[引用频次：0 ] |[阅读次数：0 ] - Jennifer L.Horton,Elena B.Samoilova,Thomas A.Judge,Laurence A.Turka,James M.Wilson
<正> Both rheumatoid arthritis and animal models ofautoimmune arthritis are characterized by hyper-activation of synovial cells and hyperplasia of the synovialmembrane. The activated synovial cells produceinflammatory cytokines and degradative enzymes whichlead to destruction of cartilage and bones. Effectivetreatment of arthritis may require elimination of most orall activated synovial cells. The death factor Fas/Apo-1
1997年03期 322页 [查看摘要][在线阅读][下载 55k] [下载次数：11 ] |[引用频次：1 ] |[阅读次数：0 ] <正> Effects of fibroblast-mediated IL-2 and IL-3 genetherapy on recovery of bone marrow depression in tumor-bearing mice treated with cyclophosphamide (300 mg/kg) and syngeneic BMT were investigated in the presentreport. BALB/c mice were inoculated with J558Lplasmacytoma cells s. c. and 3 days later IL-2 secretingNIH3T3-IL-2, IL-3 secreting NIH3T3-IL-3 fibroblastcells were implanted into the tumor-bearing mice after
1997年03期 322-323页 [查看摘要][在线阅读][下载 108k] [下载次数：11 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> During the past ten years. our molecular studies haddemonstrated that myelodysplasia of MDS bone marrowwas associated with the rearrangement/amplification ofc-erbB and deletion/ inactivation of c-erbA and identifiedthe sites of the rearrangement by v-erbB PCR genediagnosis of MDS and confirmed their etiogenicsignificance by the follow-up of MDS cases. These resultsprovided 2 target sequences of c-erbB.The antisense
1997年03期 323-324页 [查看摘要][在线阅读][下载 106k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Purpose: To explore whether the antisense RNA ofMHC-Ⅱ gene could inodulate the expression of HLA-DRgene and reduce the immunogenicity of cord blood (CB)cells.The authors transduced the antisense RNA ofMHC-Ⅱ gene into CD34 CB stem/progenitor cells.Methods: A retroviral vector encoding antisense RNA ofHLA-DR gene was constructed by molecular cloningtechnique and transducted into packaging cells line PA317by lipofectin. The high titer, helper-free, virus
1997年03期 323页 [查看摘要][在线阅读][下载 54k] [下载次数：13 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Purpose: To explore whether the antisense RNA ofMHC-Ⅱ gene could inodulate the expression of HLA-DRgene and reduce the immunogenicity of cord blood (CB)cells.The authors transduced the antisense RNA ofMHC-Ⅱ gene into CD34 CB stem/progenitor cells.Methods: A retroviral vector encoding antisense RNA ofHLA-DR gene was constructed by molecular cloningtechnique and transducted into packaging cells line PA317by lipofectin. The high titer, helper-free, virus
1997年03期 323页 [查看摘要][在线阅读][下载 54k] [下载次数：13 ] |[引用频次：0 ] |[阅读次数：0 ] <正> During the past ten years. our molecular studies haddemonstrated that myelodysplasia of MDS bone marrowwas associated with the rearrangement/amplification ofc-erbB and deletion/ inactivation of c-erbA and identifiedthe sites of the rearrangement by v-erbB PCR genediagnosis of MDS and confirmed their etiogenicsignificance by the follow-up of MDS cases. These resultsprovided 2 target sequences of c-erbB.The antisense
1997年03期 323-324页 [查看摘要][在线阅读][下载 106k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> To demonstrate that low c-myc expression mightexert the effects on differentiation and survival ofleukemic cells, antisense technique was used. Human 2. 7kb c-myc DNA fragment containing exon l, intron 1 and127nt exon 2 was ligated into retroviral vector pDOR-neoin reverse direction. This recombinant plasmid pDAC
1997年03期 324页 [查看摘要][在线阅读][下载 53k] [下载次数：15 ] |[引用频次：0 ] |[阅读次数：0 ] <正> In the present study, human IL-2 gene wastransferred into human erythroleukemic cell line K562 bythe retroviral vector LYCN. The effects on theproliferative activity of the IL-2-gene-modified K562 cells(K562/IL2) were studied. 18 Days after transduction,flow cytometry analysis indicated that (K562/IL2)accumulated at phase G_2. By day 30, the portion ofK562/IL2 was low at G_0/G_1, but still high at G_2. The
1997年03期 324页 [查看摘要][在线阅读][下载 53k] [下载次数：12 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Previous reports indicated that the difference ofMHC-Ⅰ and Ⅱ between donor and recipient was criticalfor the occurrence of allogenic rejection or graft versushost reaction (GVHR). This study aimed at looking forthe possibility of reducing the risk of immunologicalrejection through transplantation tolerance induced bytransduction of MHC gene. In the present study, wetransferred donor mice MHC class I gene (H-2k~b gene)
1997年03期 324-325页 [查看摘要][在线阅读][下载 107k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] <正> In the present study, human IL-2 gene wastransferred into human erythroleukemic cell line K562 bythe retroviral vector LYCN. The effects on theproliferative activity of the IL-2-gene-modified K562 cells(K562/IL2) were studied. 18 Days after transduction,flow cytometry analysis indicated that (K562/IL2)accumulated at phase G_2. By day 30, the portion ofK562/IL2 was low at G_0/G_1, but still high at G_2. The
1997年03期 324页 [查看摘要][在线阅读][下载 53k] [下载次数：12 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> To demonstrate that low c-myc expression mightexert the effects on differentiation and survival ofleukemic cells, antisense technique was used. Human 2. 7kb c-myc DNA fragment containing exon l, intron 1 and127nt exon 2 was ligated into retroviral vector pDOR-neoin reverse direction. This recombinant plasmid pDAC
1997年03期 324页 [查看摘要][在线阅读][下载 53k] [下载次数：15 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Previous reports indicated that the difference ofMHC-Ⅰ and Ⅱ between donor and recipient was criticalfor the occurrence of allogenic rejection or graft versushost reaction (GVHR). This study aimed at looking forthe possibility of reducing the risk of immunologicalrejection through transplantation tolerance induced bytransduction of MHC gene. In the present study, wetransferred donor mice MHC class I gene (H-2k~b gene)
1997年03期 324-325页 [查看摘要][在线阅读][下载 107k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Factor Ⅷ deficiency or hemophilia A is X-linkedgenetic disorder in human. General treatment of severehemophilia A consists of adiministration of plasma-derived or recombinant clotting factor concentrates. Ithas caused a series of problems, i. e. viral infection andcost too much to use rF Ⅷ. Nowadays, people havedeveloped the retroviral vector and the adcnoviral vector
1997年03期 325页 [查看摘要][在线阅读][下载 55k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Anemia is an invariable symptom of end-stage renalfailure. Erythropoietin (Epo) has been used to improvethe quality of life of patients with end-stage renal failure,but repeated injection of Epo requires patients frequenthospital visits and easily spreads the infectious diseases.In order to investigate whether Epo gene therapy iseffective to renal anemia, we conduct this research work.Firstly, the retrovirus vector (pLEpoSN) containing
1997年03期 325-326页 [查看摘要][在线阅读][下载 112k] [下载次数：8 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Anemia is an invariable symptom of end-stage renalfailure. Erythropoietin (Epo) has been used to improvethe quality of life of patients with end-stage renal failure,but repeated injection of Epo requires patients frequenthospital visits and easily spreads the infectious diseases.In order to investigate whether Epo gene therapy iseffective to renal anemia, we conduct this research work.Firstly, the retrovirus vector (pLEpoSN) containing
1997年03期 325-326页 [查看摘要][在线阅读][下载 112k] [下载次数：8 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Factor Ⅷ deficiency or hemophilia A is X-linkedgenetic disorder in human. General treatment of severehemophilia A consists of adiministration of plasma-derived or recombinant clotting factor concentrates. Ithas caused a series of problems, i. e. viral infection andcost too much to use rF Ⅷ. Nowadays, people havedeveloped the retroviral vector and the adcnoviral vector
1997年03期 325页 [查看摘要][在线阅读][下载 55k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Liposomes have several advantages over viral vectorsfor gene delivery both in vitro and in vivo. However,few data are available concerning gene transfer intohematopoietic stem cells. In order to explore theefficiency and the stability of expression of gene transferinto hematopoietic stem cells, we have transduced twomarker genes (Neo~R and Lac Z) co-transfer into humanbone marrow CD34~+ hematopoietic stem cells mediated
1997年03期 326页 [查看摘要][在线阅读][下载 57k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Antitumor chemotherapy is often limited by hematopoietic toxicity.TO attenuate themyelosuppressive effects of chemotherapy, successfulengraftment of unmodified fetal liver cells (FLCs) hasbeen achieved across allogeneic barriers in a number ofanimal models and patients, GM-CSF is a hematopoietic
1997年03期 326-327页 [查看摘要][在线阅读][下载 111k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Liposomes have several advantages over viral vectorsfor gene delivery both in vitro and in vivo. However,few data are available concerning gene transfer intohematopoietic stem cells. In order to explore theefficiency and the stability of expression of gene transferinto hematopoietic stem cells, we have transduced twomarker genes (Neo~R and Lac Z) co-transfer into humanbone marrow CD34~+ hematopoietic stem cells mediated
1997年03期 326页 [查看摘要][在线阅读][下载 57k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Antitumor chemotherapy is often limited by hematopoietic toxicity.TO attenuate themyelosuppressive effects of chemotherapy, successfulengraftment of unmodified fetal liver cells (FLCs) hasbeen achieved across allogeneic barriers in a number ofanimal models and patients, GM-CSF is a hematopoietic
1997年03期 326-327页 [查看摘要][在线阅读][下载 111k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Adenoviruses harboring E. coli. cytosine deaminase(CD) gene (Ad-CD) and murine granulocyte-macrophage colony stimulating factor (GM-CSF) gene(Ad-GM-CSF) were used for gene transfer in vivo.(C57BL/6 mice were inoculated subeutaneously with FBL-3 erythroleukemia cells and three days later treated withadenovirus injection at the site of tumor inoculation.The
1997年03期 327页 [查看摘要][在线阅读][下载 54k] [下载次数：7 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Stem cell factor (SCF) is a novel growth factor thatinfluences the growth and development of hematopoieticcells, germ cells and melanocytes. To explore the
1997年03期 327-328页 [查看摘要][在线阅读][下载 111k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Adenoviruses harboring E. coli. cytosine deaminase(CD) gene (Ad-CD) and murine granulocyte-macrophage colony stimulating factor (GM-CSF) gene(Ad-GM-CSF) were used for gene transfer in vivo.(C57BL/6 mice were inoculated subeutaneously with FBL-3 erythroleukemia cells and three days later treated withadenovirus injection at the site of tumor inoculation.The
1997年03期 327页 [查看摘要][在线阅读][下载 54k] [下载次数：7 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Stem cell factor (SCF) is a novel growth factor thatinfluences the growth and development of hematopoieticcells, germ cells and melanocytes. To explore the
1997年03期 327-328页 [查看摘要][在线阅读][下载 111k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] - K.Kurachi
<正> Myoblast-mediated gene transfer approach has agreat potential to be developed into a durable genetherapy method for various diseases. To increase theexpression level of human factor Ⅸ (hFⅨ) in musclecells, we constructed a series of hFⅨ expression vectorswith the retroviral vector frame and examined their hFⅨexpression in skeletal muscle cells. Firstly, we introducedhFⅨ minigene (hFⅨ ml or hFⅨ m2), which contains atruncated first intron (1. 4 kb or 0. 3 kb) of hFⅨ gene
1997年03期 328页 [查看摘要][在线阅读][下载 57k] [下载次数：11 ] |[引用频次：0 ] |[阅读次数：0 ] <正> To elucidate the feasibility of retroviral mediatedtransfer of multidrug resistance gene (mdrl) intohematopoietic stem/progenitor cells, the CD34~+ cellswere isolated from bone marrow by using a high-gradient.magnetic cell sorting system, and the efficiency ofretroviral mediated gene transfer was studied. Materials
1997年03期 328-329页 [查看摘要][在线阅读][下载 110k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] - K.Kurachi
<正> Myoblast-mediated gene transfer approach has agreat potential to be developed into a durable genetherapy method for various diseases. To increase theexpression level of human factor Ⅸ (hFⅨ) in musclecells, we constructed a series of hFⅨ expression vectorswith the retroviral vector frame and examined their hFⅨexpression in skeletal muscle cells. Firstly, we introducedhFⅨ minigene (hFⅨ ml or hFⅨ m2), which contains atruncated first intron (1. 4 kb or 0. 3 kb) of hFⅨ gene
1997年03期 328页 [查看摘要][在线阅读][下载 57k] [下载次数：11 ] |[引用频次：0 ] |[阅读次数：0 ] <正> To elucidate the feasibility of retroviral mediatedtransfer of multidrug resistance gene (mdrl) intohematopoietic stem/progenitor cells, the CD34~+ cellswere isolated from bone marrow by using a high-gradient.magnetic cell sorting system, and the efficiency ofretroviral mediated gene transfer was studied. Materials
1997年03期 328-329页 [查看摘要][在线阅读][下载 110k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Previous studies have shown that the bcl-2protooncogene encodes a mitochondrial protein thatpromotes cell survival by blocking programmed celldeath. High levels of bcl-2 expression were found inmurine myeloid leukemic cell lines resistant to apoptosisinduced by variety of agents. In addition artificialelevation of bcl-2 expression in a human pre-B leukemiccell line resulted in enhanced resistance to chemo-therapcutic drugs. We reported here on the effect ofbcl-2 antisense phosphorothiate oligodeoxynucleotides
1997年03期 329页 [查看摘要][在线阅读][下载 54k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Chemotherapy is one of the curative treatmentmodality for several types of tumors. However, thedosage of antitumor drugs was limited by cytotoxicityof drugs to normal tissues, especially for bone marrow(myelosuppression). Drug resistantce gene (hMDRI,hDHFR etc.) is expressed in many normal tissues. Itsover-expression in tumor cells give rise to the tumorresistant to many drugs. But, if we transfer the drugresistance gene into the normal hematopoietic stemcell (HSC), the expression of the foreign gene may
1997年03期 329-330页 [查看摘要][在线阅读][下载 108k] [下载次数：12 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Previous studies have shown that the bcl-2protooncogene encodes a mitochondrial protein thatpromotes cell survival by blocking programmed celldeath. High levels of bcl-2 expression were found inmurine myeloid leukemic cell lines resistant to apoptosisinduced by variety of agents. In addition artificialelevation of bcl-2 expression in a human pre-B leukemiccell line resulted in enhanced resistance to chemo-therapcutic drugs. We reported here on the effect ofbcl-2 antisense phosphorothiate oligodeoxynucleotides
1997年03期 329页 [查看摘要][在线阅读][下载 54k] [下载次数：9 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Chemotherapy is one of the curative treatmentmodality for several types of tumors. However, thedosage of antitumor drugs was limited by cytotoxicityof drugs to normal tissues, especially for bone marrow(myelosuppression). Drug resistantce gene (hMDRI,hDHFR etc.) is expressed in many normal tissues. Itsover-expression in tumor cells give rise to the tumorresistant to many drugs. But, if we transfer the drugresistance gene into the normal hematopoietic stemcell (HSC), the expression of the foreign gene may
1997年03期 329-330页 [查看摘要][在线阅读][下载 108k] [下载次数：12 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> In order to increase the expression of mdrl genetransfectcd using retroviral vcctors, we constructedtwo retroviral vectors (LmdrlSN and MSCV-mdrl)containing mdrl cDNA and compared the expressionof mdrl gene with another vector pHamdrl/A(Pastan et al. Proc Natl Acad Sci USA 85: 4486,1986) in NIH.3T3 cells. The LmdrlSN was made by
1997年03期 330-331页 [查看摘要][在线阅读][下载 104k] [下载次数：8 ] |[引用频次：0 ] |[阅读次数：0 ] <正> In order to increase the expression of mdrl genetransfectcd using retroviral vcctors, we constructedtwo retroviral vectors (LmdrlSN and MSCV-mdrl)containing mdrl cDNA and compared the expressionof mdrl gene with another vector pHamdrl/A(Pastan et al. Proc Natl Acad Sci USA 85: 4486,1986) in NIH.3T3 cells. The LmdrlSN was made by
1997年03期 330-331页 [查看摘要][在线阅读][下载 104k] [下载次数：8 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> In order to explore whether the rearrangement and amplification of C-erbB-l gene were associatedwith the development of MDS and their clinicalsignificance in diagnoses and prognoses. digitoxin-labelled oligonucleotide probes were used to detect 33
1997年03期 331页 [查看摘要][在线阅读][下载 49k] [下载次数：11 ] |[引用频次：0 ] |[阅读次数：0 ] <正> We have constructed three recombinantadenoviruses encoding RA538, p53 or p16 respec-tively. These recombinant adenoviruses are able toexpress RA538, p53 or p16 in infected human cancercells. The transduction efficiency assay showed thatthe infected cancer cells inoculated with theserecombinant adenoviruses at MOI of 25 or greaterexhibited 100% transduction efficiency. The growthrates and colony formation of cancer cells infectedwith these recombinant adenoviruses were greatly
1997年03期 331-332页 [查看摘要][在线阅读][下载 103k] [下载次数：12 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> In order to explore whether the rearrangement and amplification of C-erbB-l gene were associatedwith the development of MDS and their clinicalsignificance in diagnoses and prognoses. digitoxin-labelled oligonucleotide probes were used to detect 33
1997年03期 331页 [查看摘要][在线阅读][下载 49k] [下载次数：11 ] |[引用频次：0 ] |[阅读次数：0 ] <正> We have constructed three recombinantadenoviruses encoding RA538, p53 or p16 respec-tively. These recombinant adenoviruses are able toexpress RA538, p53 or p16 in infected human cancercells. The transduction efficiency assay showed thatthe infected cancer cells inoculated with theserecombinant adenoviruses at MOI of 25 or greaterexhibited 100% transduction efficiency. The growthrates and colony formation of cancer cells infectedwith these recombinant adenoviruses were greatly
1997年03期 331-332页 [查看摘要][在线阅读][下载 103k] [下载次数：12 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Objective: In order to coordinately express amarker gene and therapeutic genes in one vector, apolycistronic retroviral vector was constructed withthe internal ribosome entry site from encephalo-myocarditis and polio virus, which was able to expressthree different genes simultaneously. Methods: Thevector used mouse moloney leukemia virus sequencesfor viral expression and packaging functions and
1997年03期 332页 [查看摘要][在线阅读][下载 54k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Human chromosomes terminate with severalkilobases of the simple telomere repeat (TTAGGG) n.Telomeres protect the chromosomes from DNAdegradation, end-to-end fusion, rearrangements andchromosome losses. Since DNA polymerasessynthesize DNA in the 5' to 3' direction and require aRNA primer for initiation, telomeric DNA could belost at chromosome ends unless the termini arespecifically extended by telomerase. Telomerase is aspecialized ribonucleoprotein polymerase that containsan integral RNA with a short template element that
1997年03期 332-333页 [查看摘要][在线阅读][下载 107k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> Objective: In order to coordinately express amarker gene and therapeutic genes in one vector, apolycistronic retroviral vector was constructed withthe internal ribosome entry site from encephalo-myocarditis and polio virus, which was able to expressthree different genes simultaneously. Methods: Thevector used mouse moloney leukemia virus sequencesfor viral expression and packaging functions and
1997年03期 332页 [查看摘要][在线阅读][下载 54k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] <正> Human chromosomes terminate with severalkilobases of the simple telomere repeat (TTAGGG) n.Telomeres protect the chromosomes from DNAdegradation, end-to-end fusion, rearrangements andchromosome losses. Since DNA polymerasessynthesize DNA in the 5' to 3' direction and require aRNA primer for initiation, telomeric DNA could belost at chromosome ends unless the termini arespecifically extended by telomerase. Telomerase is aspecialized ribonucleoprotein polymerase that containsan integral RNA with a short template element that
1997年03期 332-333页 [查看摘要][在线阅读][下载 107k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] - Sudhir Agrawal
<正> Antisense oligonucleotides have been shown to beunique drugs that achieve their effect by targetingmRNA with which they can hybridize and specificallyblock gene expression, offering the possibility ofspecific, rational drug design. However, there havebeen many concerns that have limited enthusiasm forthe development of these drugs from the preclinical to
1997年03期 333-334页 [查看摘要][在线阅读][下载 107k] [下载次数：6 ] |[引用频次：0 ] |[阅读次数：0 ] - Sudhir Agrawal
<正> Antisense oligonucleotides have been shown to beunique drugs that achieve their effect by targetingmRNA with which they can hybridize and specificallyblock gene expression, offering the possibility ofspecific, rational drug design. However, there havebeen many concerns that have limited enthusiasm forthe development of these drugs from the preclinical to
1997年03期 333-334页 [查看摘要][在线阅读][下载 107k] [下载次数：6 ] |[引用频次：0 ] |[阅读次数：0 ] - Hirofumi Hamada
<正> Interleukin-2(IL-2) has been demonstrated to beone of the most effective target genes in cancerimmunogene therapy. There are more than 20 clinicalprotocols of cancer gene therapy introducing IL-2 intotumor patients to treat melanoma, renal carcinoma,prostate carcinoma, colon carcinoma, lung
1997年03期 334页 [查看摘要][在线阅读][下载 53k] [下载次数：8 ] |[引用频次：0 ] |[阅读次数：0 ] - Hirofumi Hamada
<正> Interleukin-12, a heterodimer produced mainlyby antigen presenting cells (APCs), plays vital rolesin the induction of Thl-mediated immune responsesand has the potential to treat cancer and infectiousdiseases. Systemic administration of IL-12 wasobserved to be associated with severe toxicity. To takefull advantage of its immunoregulatory activities andminimize its toxicities, local administration of IL-12may be the ideal approach. Intratumoral injection of
1997年03期 334-335页 [查看摘要][在线阅读][下载 111k] [下载次数：13 ] |[引用频次：0 ] |[阅读次数：0 ] - Hirofumi Hamada
<正> Interleukin-2(IL-2) has been demonstrated to beone of the most effective target genes in cancerimmunogene therapy. There are more than 20 clinicalprotocols of cancer gene therapy introducing IL-2 intotumor patients to treat melanoma, renal carcinoma,prostate carcinoma, colon carcinoma, lung
1997年03期 334页 [查看摘要][在线阅读][下载 53k] [下载次数：8 ] |[引用频次：0 ] |[阅读次数：0 ] - Hirofumi Hamada
<正> Interleukin-12, a heterodimer produced mainlyby antigen presenting cells (APCs), plays vital rolesin the induction of Thl-mediated immune responsesand has the potential to treat cancer and infectiousdiseases. Systemic administration of IL-12 wasobserved to be associated with severe toxicity. To takefull advantage of its immunoregulatory activities andminimize its toxicities, local administration of IL-12may be the ideal approach. Intratumoral injection of
1997年03期 334-335页 [查看摘要][在线阅读][下载 111k] [下载次数：13 ] |[引用频次：0 ] |[阅读次数：0 ] - Hirofumi Hamada
<正> Granulocyte/macrophage colony-stimulatingfactor (GM-CSF) plays important roles in theregulation of hematogenesis and the immuneresponses. Vaccination with GM-CSF gene-modifiedtumor cells was demonstrated to be capable ofinducing long-lasting, effective and specific antitumorimmune reaction. This protocol has entered clinical
1997年03期 335-336页 [查看摘要][在线阅读][下载 104k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] - Hirofumi Hamada
<正> Granulocyte/macrophage colony-stimulatingfactor (GM-CSF) plays important roles in theregulation of hematogenesis and the immuneresponses. Vaccination with GM-CSF gene-modifiedtumor cells was demonstrated to be capable ofinducing long-lasting, effective and specific antitumorimmune reaction. This protocol has entered clinical
1997年03期 335-336页 [查看摘要][在线阅读][下载 104k] [下载次数：10 ] |[引用频次：0 ] |[阅读次数：0 ] - J.H.Zhu,L.Zhang,R.Reszka
<正> The development of improved gene transfermethods is a prerequisite for gene therapy to realizeits full potentials. One approach is the design ofplasmid-based delivery system that also termed "self-assembling complexes" such as cationic liposome-DNAcomplex (lipoplex) and protein-DNA complex.Unlike viral vectors, liposome-DNA complexes arenoninfectious, nonimmunogenic and exhibit low in
1997年03期 336页 [查看摘要][在线阅读][下载 46k] [下载次数：7 ] |[引用频次：0 ] |[阅读次数：0 ] <正> The CDKN2 (MTS1/P16~(INK4A)) is believed as atumor suppressor gene. It maps in the human'schromosome gp21. It encodes a p16~(INK4A) protein thatis an inhibitor of cyclin-dependent kinase 4. CDKN2gene's homozygous deletion is common in many tumorderived cell lines. Purpose: We examine whether
1997年03期 336页 [查看摘要][在线阅读][下载 46k] [下载次数：7 ] |[引用频次：0 ] |[阅读次数：0 ] -
<正> The CDKN2 (MTS1/P16~(INK4A)) is believed as atumor suppressor gene. It maps in the human'schromosome gp21. It encodes a p16~(INK4A) protein thatis an inhibitor of cyclin-dependent kinase 4. CDKN2gene's homozygous deletion is common in many tumorderived cell lines. Purpose: We examine whether
1997年03期 336页 [查看摘要][在线阅读][下载 46k] [下载次数：7 ] |[引用频次：0 ] |[阅读次数：0 ] - J.H.Zhu,L.Zhang,R.Reszka
<正> The development of improved gene transfermethods is a prerequisite for gene therapy to realizeits full potentials. One approach is the design ofplasmid-based delivery system that also termed "self-assembling complexes" such as cationic liposome-DNAcomplex (lipoplex) and protein-DNA complex.Unlike viral vectors, liposome-DNA complexes arenoninfectious, nonimmunogenic and exhibit low in
1997年03期 336页 [查看摘要][在线阅读][下载 46k] [下载次数：7 ] |[引用频次：0 ] |[阅读次数：0 ] 下载本期数据